Strain Information
| Name | EU3020 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | cls-2(or1948)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. |
| Description | qIs26 [lag-2::GFP + rol-6(su1006)]. or1948 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1948 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Aleesa Schlientz |
| Laboratory | EU |
| Reference | Schlientz AJ, Bowerman B. C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. 2020. |
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