Strain Information

Name CZ24092   View On Wormbase
Species C. elegans
Genotypegip-2(lt19[gip-2::GFP::loxP::Cbr-unc-119(+)::loxP]) I; ltSi953 II; unc-119(ed3) III.
DescriptionltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous gip-2 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged gip-2 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826.
MutagenNo mutagen
Outcrossedx0
Made byNgang Heok Tang
Laboratory CZ
Reference Shaohe Wang, Ngang Heok Tang, Pablo Lara-Gonzalez, Zhiling Zhao, Dhanya K Cheerambathur, Bram Prevo, Andrew D Chisholm, Arshad Desai, Karen Oegema. A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans. Development. 2017 Jul 15;144(14):2694-2701. doi: 10.1242/dev.150094. Epub 2017 Jun 15.
Sign in or register an account if you want to order this strain.