Strain Information
| Name | CGC183 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | mir-1829.1(umn88[mir-1829.1p+SL1::EGL13NLS::lox2272])/tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X. |
| Description | tmIs1240 [myo-2p::venus, X: F23D12.4] X. Deletion of mir-1829.1 pre-miRNA. A cassette containing mScarlet-I and an SEC flanked by lox2272 was introduced into the mir-1829.1 loci using CRISPR/Cas9. This line was generated by excising mScarlet-I and the SEC leaving a SL1::EGL13NLS::lox2272 scar. Heterozygotes are wild-type GFP+ and segregate wild-type GFP+ heterozygotes, non-GFP arrested larvae (umn88 homozygotes) and Mec(Unc) GFP+ (tmC24 homozygotes). Maintain by picking wild-type GFP+. Left Flanking: TGCTAAATCCTTTGTTCAGTTATTATAAGT, Right Flanking: ACATTTTTGGATTGGCAAAGTTGTAGTACT. sgRNA: GTTCAGTTATTATAAGTAAG. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Julie Knott and Marcus L. Vargas |
| Laboratory | CGC |
| Reference | n/a |
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