Strain Information
| Name | CG1490 View On Wormbase |
|---|---|
| Species | C.elegans |
| Genotype | pck-2(rg551) I; him-5(e1490) V; sdha-1(rg550) X. |
| Description | Slow growing (4 to 7 days from L1 to adult), sluggish movement, egg-laying defective, short lifespan (~5 to 6 days adult lifespan); male mating impotent. sdha-1(rg550) is viable and likely a partial loss-of-function allele; the sdha-1(ok1908) deletion is lethal. sdha-1(rg550) is a CRISPR/Cas9 + oligo-induced gene conversion into the sdha-1 gene recapitulating a G214E substitution originally identified in an EMS mutant screen. The lesion was recreated via CRISPR/Cas9 in N2 to ensure the mutation/phenotype was not linked to other background mutations induced during mutatgenesis. rg551 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the gene pck-2 in N2 background. YFP fluorescence body wall muscle, intestine, hypodermis and pharyngeal muscle. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x3 |
| Made by | L. Rene Garcia |
| Laboratory | CG |
| Reference | Goncalves, J. et al. Succinate Dehydrogenase-Regulated Phosphoenolpyruvate Carboxykinase Sustains Copulation Fitness in Aging C. elegans Males. iScience 23, 100990 (2020). |
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