Laboratory Information

NameCG View on WormBase
Allele designationrg
HeadGarcia, Rene
InstitutionTexas A&M University, College Station, TX
Address Department of Biology
Texas A&M University
3258 TAMU
College Station 77843
United States
Website http://www.bio.tamu.edu/USERS/Garcia/index.html
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
BZ1202 seb-3(eg696) X. C. elegans This strain is tolerant to acute treatment of ethanol. The severity and incidence of stress-induced tremors are greater than in wild-type. Reference: Jee C, et al. Genes Brain Behav. 2013 Mar;12(2):250-62.
CG1 lev-11(rg1) I; him-5(e1490) V. C. elegans Resistant to levamisole. Egl-d. Twitch at 25C. Long. Throws males.
CG118 unc-43(sy574) IV; him-5(e1490) V. C. elegans Males constitutively protract their spicules in the absence of mating cues.
CG1367 pck-2(rg551) I; him-5(e1490) V. C.elegans rg551 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the gene pck-2 in N2 background. Robust YFP fluorescence body wall muscle, intestine, hypodermis and pharyngeal muscle. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955
CG1428 tmc-1(rg1003) X. C. elegans rg1003 promotes development and male mating behavior on a chemically defined C. elegans maintenance medium (CeMM). Reference: Zhang L, et al. Nature Communications (2015) In press.
CG1438 egl-2(rg444) him-5(e1490) V. C. elegans rg444 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the EGL-2 K+ Channel in N2 background. Faint YFP fluorescent puncta can be detected on muscle membranes and neural cell bodies and processes at high power magnification in all stages. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955
CG1490 pck-2(rg551) I; him-5(e1490) V; sdha-1(rg550) X. C.elegans Slow growing (4 to 7 days from L1 to adult), sluggish movement, egg-laying defective, short lifespan (~5 to 6 days adult lifespan); male mating impotent. sdha-1(rg550) is viable and likely a partial loss-of-function allele; the sdha-1(ok1908) deletion is lethal. sdha-1(rg550) is a CRISPR/Cas9 + oligo-induced gene conversion into the sdha-1 gene recapitulating a G214E substitution originally identified in an EMS mutant screen. The lesion was recreated via CRISPR/Cas9 in N2 to ensure the mutation/phenotype was not linked to other background mutations induced during mutatgenesis. rg551 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the gene pck-2 in N2 background. YFP fluorescence body wall muscle, intestine, hypodermis and pharyngeal muscle. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955
CG197 egl-2(rg4) him-5(e1490) V. C. elegans No longer able to suppress male muscle seizures in the absence of food.
CG21 egl-30(tg26) I; him-5(e1490) V. C. elegans Hyperactive. Small. Spicule protruder. Coiler. Throws males.
CG289 unc-103(n1213) pha-1(e2123) III; him-5(e1490) V;rgEx81. C. elegans rgEx81[pha-1(+) + unc-103 promoter E::unc-103 genomic DNA]. Maintain at 20C.
CG460 unc-43(sy574) IV; him-5(e1490) V; rgEx161. C. elegans rgEx161[lev-11p::UNC-43g + gtl-1p::CFP]. rgEx161 rescues unc-43(sy574)-induced spicule protraction.
CG490 unc-103(n1213) III; egl-2(rg4) him-5(e1490) V; rgEx173. C. elegans rgEx173 [unc-103ep::egl-2(cDNA) + gtl-1p::CFP]. Pick animals with cyan fluorescence in their intestines. rgEx173 rescues food deprivation's ability to suppress unc-103(n1213)-induced male sex muscle seizures.
CG499 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. C. elegans rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction.
CG500 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. C. elegans rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation.
CG625 unc-103(n1213) pha-1(e2123) III; him-5(e1490) V; rgEx235. C. elegans rgEx235 [plc-3p::YFP + pha-1(+)]. Expresses YFP from a 4.4bk upstream plc-3 promoter. Maintain at 20C.
CG640 daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. C. elegans rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction.
CG644 pha-1(e2123) III; him-5(e1490) V; rgEx251. C. elegans rgEx251[pha-1(+) + osm-12 promoter:: unc-103(gf)]. Maintain at 20C.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
rg1 Allele substitution
rg1003 Allele substitution
rg4 Allele deletion