Laboratory Information
| Name | CG View on WormBase |
|---|---|
| Allele designation | rg |
| Head | Garcia, Rene |
| Institution | Texas A&M University, College Station, TX |
| Address | Department of Biology Texas A&M University 3258 TAMU College Station 77843 United States |
| Website | http://www.bio.tamu.edu/USERS/Garcia/index.html |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BZ1202 | seb-3(eg696) X. | C. elegans | This strain is tolerant to acute treatment of ethanol. The severity and incidence of stress-induced tremors are greater than in wild-type. Reference: Jee C, et al. Genes Brain Behav. 2013 Mar;12(2):250-62. |
| CG1 | lev-11(rg1) I; him-5(e1490) V. | C. elegans | Resistant to levamisole. Egl-d. Twitch at 25C. Long. Throws males. |
| CG118 | unc-43(sy574) IV; him-5(e1490) V. | C. elegans | Males constitutively protract their spicules in the absence of mating cues. |
| CG1367 | pck-2(rg551[pck-2::YFP]) I; him-5(e1490) V. | C.elegans | rg551 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the gene pck-2 in N2 background. Robust YFP fluorescence body wall muscle, intestine, hypodermis and pharyngeal muscle. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955 |
| CG1428 | tmc-1(rg1003) X. | C. elegans | rg1003 promotes development and male mating behavior on a chemically defined C. elegans maintenance medium (CeMM). Reference: Zhang L, et al. Nature Communications (2015) In press. |
| CG1438 | egl-2(rg444) him-5(e1490) V. | C. elegans | rg444 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the EGL-2 K+ Channel in N2 background. Faint YFP fluorescent puncta can be detected on muscle membranes and neural cell bodies and processes at high power magnification in all stages. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955 |
| CG197 | egl-2(rg4) him-5(e1490) V. | C. elegans | No longer able to suppress male muscle seizures in the absence of food. |
| CG21 | egl-30(tg26) I; him-5(e1490) V. | C. elegans | Hyperactive. Small. Spicule protruder. Coiler. Throws males. |
| CG289 | unc-103(n1213) pha-1(e2123) III; him-5(e1490) V;rgEx81. | C. elegans | rgEx81[pha-1(+) + unc-103 promoter E::unc-103 genomic DNA]. Maintain at 20C. |
| CG460 | unc-43(sy574) IV; him-5(e1490) V; rgEx161. | C. elegans | rgEx161[lev-11p::UNC-43g + gtl-1p::CFP]. rgEx161 rescues unc-43(sy574)-induced spicule protraction. |
| CG490 | unc-103(n1213) III; egl-2(rg4) him-5(e1490) V; rgEx173. | C. elegans | rgEx173 [unc-103ep::egl-2(cDNA) + gtl-1p::CFP]. Pick animals with cyan fluorescence in their intestines. rgEx173 rescues food deprivation's ability to suppress unc-103(n1213)-induced male sex muscle seizures. |
| CG499 | daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. | C. elegans | rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction. |
| CG500 | daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. | C. elegans | rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation. |
| CG625 | unc-103(n1213) pha-1(e2123) III; him-5(e1490) V; rgEx235. | C. elegans | rgEx235 [plc-3p::YFP + pha-1(+)]. Expresses YFP from a 4.4bk upstream plc-3 promoter. Maintain at 20C. |
| CG640 | daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. | C. elegans | rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction. |
| CG644 | pha-1(e2123) III; him-5(e1490) V; rgEx251. | C. elegans | rgEx251[pha-1(+) + osm-12 promoter:: unc-103(gf)]. Maintain at 20C. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| rg1 | Allele | substitution | |
| rg1003 | Allele | substitution | |
| rg4 | Allele | deletion |