Strain Information

Name CER660   View On Wormbase
Species C. elegans
Genotypecer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
DescriptionMissense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4.
MutagenCrispr/Cas9
Outcrossedx0
Made byDmytro Kukhtar
Laboratory CER
Reference Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes. Jeremy Vicencio, Carlos Sánchez-Bolaños,et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4.
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