Strain Information
| Name | CER660 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III. |
| Description | Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Dmytro Kukhtar |
| Laboratory | CER |
| Reference | Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes. Jeremy Vicencio, Carlos Sánchez-Bolaños,et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. |
Sign in
or
register an account if you want to order this strain.