| BA1013 |
spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. |
C. elegans |
Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line. |
| BA1061 |
dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal. |
| BG99 |
laf-1(q267)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and larval lethals (laf-1 homozygotes). Maintain by picking WT. |
| BN464 |
bqSi189 II; mel-28(t1684) unc-32(e189)/qC1 dpy-19(e1259) glp-1(q339) III; ojIs1 |
C. elegans |
bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 25C to help prevent silencing of ojIs1. mel-28 heterozygotes are wild-type and segregate wild-type, DpySteriles, and Uncs which give only dead eggs. ojIs1 is likely integrated into LG V. bqSi189 is a single-copy MosSCI insertion into ttTi5605 derived from injection of pBN13. Derived from GE2622; this strain might still carry him-3(e1147) in the background. Reference: Gomez-Saldivar G, et al. PLoS Genet. 2016 Jun 24;12(6):e1006131. |
| BW1369 |
unc-32(e189) dpy-18(e364) ctDf2/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. |
| BW1535 |
dpy-18(e364) ctDf3 unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. |
| BW1563 |
pal-1(ct281)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct281 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct281 is a 4.7kb deletion removing intron 5, exon 6, and the 3'UTR of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. |
| BW1566 |
pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. |
| BW1634 |
nob-1(ct223)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
WT hermaphrodites that segregate WT, Dpy Steriles and Nob larvae. The Nob phenotype results in 100% lethality. Maintain by picking WT. |
| BW1927 |
pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III; ctIs33. |
C. elegans |
ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. |
| CB1259 |
dpy-19(e1259) III. |
C. elegans |
Temperature sensitive Dpy. Reproduction poor at 25C. M-MATING+ AT LOW TEMPERATURE ONLY. |
| CB2195 |
dpy-19(e1259) unc-32(e189) III. |
C. elegans |
Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C. |
| CB4681 |
nDf17/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are Dpy and segregate Dpy, Sterile Dpys and dead eggs. [CGC received new stock in 1/1999 from Leon Avery.] |
| CH1179 |
unc-36(e251) emb-9(g23cg46)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles and 3-fold lethals. cg46 is a 497 bp deletion that removes the last 22 nucleotides of intron 9 and 475 nucleotides of exon 10; |
| CH1180 |
unc-32(e189) emb-9(cg56)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles and Uncs which arrest in the L1 stage. |
| CV98 |
him-18(tm2181)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. |
C. elegans |
qIs26 [lag-2::GFP + rol-6(su1006)]. Heterozygous animals show roller phenotype and GFP signal at the distal tip cells. Segregates roller GFP(+) heterozygotes, wild-type moving GFP(-) him-18(tm2181) homozygotes. qC1 [dpy-19(e1259) glp-1(q339) qIs26] homozygous animals are dead. P0 him-18(tm2181) homozygous animals show 80% embryonic lethality and 12% high incidence of male at F1. Pick roller GFP(+) worms to maintain. Reference: Saito TT, et al. (2009) PLoS Genet 5:e1000735. |
| DA686 |
nDf16/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. dpy-19(e1259) is temperature sensitive. Maintain by picking WT. [Checked 11/94 with sma-3 and nDf16 is present.] |
| DR1410 |
dpy-27(y56)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
WT strain which segregates WT, Dpy and DpySteriles (or WT Steriles if grown at 15C.) Maintain by picking WT. dpy-27 animals are mildly Dpy and Egl with protruding vulva. Maternal effect: progeny of dpy-27 homozygotes will be severely Dpy, marginally viable, and their progeny will be more severe Dpy hermaphrodites and WT males. Males are vigorous and mate well. See also WBPaper00002061. |
| DV3462 |
rheb-1(re242)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III. |
C. elegans |
nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complement all markers on qC1. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+ (pharynx), and segregate wild-type GFP+ heterozygotes, Dpy Sterile GFP+, and non-GFP re242 homozygotes (arrested at L3). |
| EL186 |
dpy-19(e1259) ego-4(om30)/eT1 III; +/eT1 V. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. dpy-19 ego-4 progeny die as embryos. |
| EL187 |
dpy-19(e1259) ego-5(om31)/eT1 III; +/eT1 V. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. |
| EU2836 |
unc-32(e189) sas-7(or452) / qC1[dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregates WT, Sterile Dpys, and Unc. Unc worms produce temperature sensitive lethal embryos with centriole duplication defects. Keep at 15˚C to maintain or452 homozygotes. Ref. Sugioka, Hamill, et al., (2016) eLife 6 e20353. |
| EU3020 |
cls-2(or1948)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. |
C. elegans |
qIs26 [lag-2::GFP + rol-6(su1006)]. or1948 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1948 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. |
| EU3023 |
cls-2(or1951)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. |
C. elegans |
qIs26 [lag-2::GFP + rol-6(su1006)]. or1951 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1951 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. |
| EU396 |
cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. |
| EU415 |
cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-8(e1489) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs at all temperatures. Throws males. |
| EU618 |
mlc-4(or253)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and late embryonic or early larval lethals with incomplete elongation/morphogenesis. |
| EU918 |
lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III; spn-4(or191) V. |
C. elegans |
Temperature sensitive spn-1. Maintain at 15C. Lethal at 25C. Heterozygotes are WT and segregate WT, Lon which give only dead eggs at all temperatures, and Sterile Dpys(ts). |
| FF275 |
dpy-17(e164) unc-32(f123) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and embryonic lethals (f123 homozygotes) |
| FF276 |
dpy-17(e164) unc-32(f121) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and larval lethals (f121 homozygotes). g to a transition in exon 6 (2874), changing a Gly in Glu in ZK637.8 gene encoding a V-ATPase alpha subunit |
| FR431 |
ceh-13(sw1)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles and ceh-13 homozygotes which are dead eggs and deformed embryos. |
| GE2158 |
tDf2/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Homozygous tDf2 is embryonic lethal. Maintain by picking WT. |
| GE2167 |
unc-32(e189) tDf5/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. |
| GE2171 |
tDf9 unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs, and males. tDf9 is embryonic lethal. |
| GE2172 |
unc-32(e189) tDf8/qC1 [dpy-19(e1259) glp-1(q339)] III. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. See WBG 13 (4): 96. |
| GE2175 |
unc-32(e189) tDf6/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. |
| GE2180 |
unc-32(e189) tDf7/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. |
| GE2198 |
cta-3(t1492) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2199 |
unc-32(e189) nup-1(t1482)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2204 |
unc-32(e189) tDf10/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and dead eggs, and males. tDf10 is embryonic lethal. |
| GE2211 |
unc-32(e189) sas-1(t1476)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2212 |
unc-32(e189) com-1(t1489)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Dpy Steriles, and Uncs which are Mel with over 99% embryonic lethality. |
| GE2219 |
unc-32(e189) csg-1(t1501)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2238 |
cyk-1(t1611) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. Throws males. |
| GE2239 |
unc-32(e189) csg-7(t1612)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2240 |
apo-3(t1613) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2242 |
cta-1(t1618) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |
| GE2244 |
unc-32(e189) lit-1(t1512)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles and Uncs which produce only dead eggs at 25C but viable progeny at 15C. Throws males. |
| GE2245 |
unc-32(e189) tim-1(t1545)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tim-1 is temperature sensitive: progeny are viable at 15C, and dead at 25C. Previously called csg-5(t1545). Received new stock 5/04. |
| GE2255 |
unc-32(e189) tbb-2(t1623)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. |
C. elegans |
Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. |