Laboratory Information

NameBW View on WormBase
Allele designationct
HeadWood, Bill
InstitutionUniversity of Colorado, Boulder
Address Department of MCD Biology University of Colorado at Boulder 347 UCB Boulder, CO 80309-0347

Website http://mcdb.colorado.edu/mcdb/wood/
Gene classes aha  ahr  cdh  dbl  gad  hbl  mag  nob  php  pun  vet  zyg 

Strains contributed by this laboratory

Strain Genotype Species Description
BW1039 dpy-5(e61) unc-40(e271) I. C. elegans DpyUnc. Slightly Egl.
BW1046 unc-37(e262) daf-8(e1393) I. C. elegans Unc. Constitutive dauer formation (Daf-c) at 25C.
BW1049 unc-40(e271) bli-4(e937) I. C. elegans UncBli Strain. Blisters late.
BW1053 dpy-14(e188) unc-13(e1091) I. C. elegans Unc. Temperature-sensitive Dpy. Grows best at 15C.
BW1084 ncl-1(e1865) unc-36(e251) tra-1(e1099) dpy-18(e364) III; ctDp6 (III;f). C. elegans Animals which carry ctDp6 are WT. Animals which have lost ctDp6 are DpyUnc Males. Maintain by picking WT.
BW1145 tra-1(e1099) dpy-18(e364) III; ctDp2 (III;f). C. elegans Animals which carry ctDp2 are WT. Animals which have lost ctDp2 are Dpy Males. Maintain by picking WT.
BW1161 unc-22(ct169) IV. C. elegans Twitcher.
BW1163 unc-22(ct171) IV. C. elegans Twitcher.
BW1166 unc-22(ct174) IV. C. elegans Twitcher.
BW1168 unc-22(ct176) IV. C. elegans Twitcher.
BW1169 unc-22(ct177) IV. C. elegans Twitcher.
BW1176 unc-22(ct181) IV. C. elegans
BW1199 him-8(e1489) IV; unc-42(e270) sma-1(e30) V; ctDp8[vab-8(e1017)] (V;f). C. elegans
BW1341 nob-1(ct223) III; eDp6 (III;f). C. elegans Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain; typically segregates about 50% wild type progeny and 50% Nob larvae.
BW1369 unc-32(e189) dpy-18(e364) ctDf2/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT.
BW1434 dpy-17(e164) III; him-8(e1489) IV; her-1(y101hv1) unc-42(e270) V; ctDp11 (III;V;f). C. elegans Wild type hermaphrodites and males which produce WT, Dpy, Unc and DpyUnc progeny due to mating or loss of the duplication. Pick WT hermaphrodites and males to maintain.
BW1535 dpy-18(e364) ctDf3 unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT.
BW1561 dpy-18(e364) nob-1(ct223) unc-25(e156) III; eDp6 (III;f). C. elegans Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain. The Dpy and Unc phenotypes are not visible in the Nob background. ct223 is recessive.
BW1563 pal-1(ct281)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct281 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct281 is a 4.7kb deletion removing intron 5, exon 6, and the 3'UTR of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
BW1566 pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
BW163 ctDf1 V/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs (heterozygotes) and dead eggs (ctDf1 homozygotes or nT1 homozygotes).
BW1634 nob-1(ct223)/qC1 [dpy-19(e1259) glp-1(q339)] III. C. elegans WT hermaphrodites that segregate WT, Dpy Steriles and Nob larvae. The Nob phenotype results in 100% lethality. Maintain by picking WT.
BW1651 nob-1(ct351) III; eDp6 (III;f). C. elegans Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain; segregates wild type progeny, Nob larvae, and occasional dead eggs. ct351 is recessive.
BW1739 +/eT1 III; unc-62(t2012) dpy-11(e224)/eT1 V. C. elegans Heterozygotes are wild-type, and segregate WT heterozygotes, Dpy Mel (unc-62 dpy-11 homozygotes), and dead eggs (eT1 homozygotes). unc-62 dpy-11 homozygotes give only dead embryos (96%) or dead larva (4%).
BW1747 dpy-18(e364)/eT1 III; unc-46(e177) let-427(s1057)/eT1 V. C. elegans Heterozygotes are WT and segregate WT, Unc-36, Sterile DpyUncs and dead eggs. Maintain by picking WT.
BW1809 gpa-16(it143) I; him-5(e1490) V. C. elegans Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs.
BW1850 Cbr-him(s1290). C. briggsae C. briggsae strain. Hermaphrodites are variably self-sterile, which is overcome by mating to males of the same genotype. From BC1991.
BW1851 ctIs33. C. elegans ctIs33 [pal-1::GFP + rol-6(su1006)]. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
BW1927 pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III; ctIs33. C. elegans ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
BW1932 ctIs39. C. elegans ctIs39 [hbl-1p::GFP::NLS::hbl-1 3'UTR + rol-6(su1006)]. Rollers. Reporter encodes the first 133 amino acids of HBL-1 followed by GFP with SV40 NLS and 1.4 kb of hbl-1 3' UTR. Reference: Fay DS, et al. Dev Biol. 1999 Jan 15;205(2):240-53.
BW1935 unc-119(ed3) III; ctIs43 him-5(e1490) V. C. elegans ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)].
BW1940 ctIs40 X. C. elegans ctIs40 [dbl-1(+) + sur-5::GFP]. Lon. dbl-1 over-expressing line from injection of constructs ZC421 and pTG98.
BW1943 gad-1(ct226) dpy-11(e224) V. C. elegans Maintain at 15C. Temperature-sensitive maternal effect, gastrulation defective. 100% of embryos arrest without gastrulating at 25C.
BW1946 ctIs43 unc-42(e270) V. C. elegans Unc. ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)].
BW2027 unc-62(ct344) V; yDp1 (IV;V;f). C. elegans Animals carrying the duplication are wild-type. Animals which have lost yDp1 are Unc and give only dead eggs(57%) or dead larvae(43%).
BW2041 pal-1(ct224) dpy-17(e164) ncl-1(e1865) unc-36(e251) III; svDp1 (III;f). C. elegans svDp1 [sur-5::GFP] (III;f). svDp1 was derived by insertion of a sur-5::GFP marker into sDp3(III;f). Pick GFP+ wild-type to maintain. svDp1 rescues ct224. ct224 homozygotes (lacking GFP expression) show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. The duplication is lost in about 30-40% of embryos. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
BW2063 ceh-20(ay38) unc-36(e251) III; svDp1 (III;f). C. elegans May have unc-4(e120) mutation in background. svDp1 balances from pal-1 through unc-36 on III. svDp1 made by fusing array containing [unc-4(+) + sur-5::GFP] to sDp3(III;f).
BW219 nDf31/unc-23(e25) sma-1(e30) V. C. elegans Heterozygotes are WT and segregate SmaUnc and Lethals. Lethal in L1. Maintain by picking WT.
BW287 Caenorhabditis briggsae. Panagrolaimus sp. Chinese rhabditis hermaphrodite nematode from Bill Wood's Chinese collection, Beijing. According to David Fitch this strain is Panagrolaimus sp. April 2008: According to sequence data from Howe & Denver 2008 BMC Evol Biol 8:62, strain BW287 is C. briggsae.
BW315 mig-10(ct41) III. C. elegans Withered tail and Egl. Adults shorter than WT. Embryonic cell migrations affected: ALM and HSN with high penetrance, CAN with moderate penetrance. All misplaced nuclei at positions indicating incomplete migration.
BW506 ceh-10(ct78) III. C. elegans Withered tail. Adults shorter than WT. Embryonic cell migrations affected: CAN migration with high penetrance. Previously called mig-11.
BW538 unc-13(e1091) lin-10(e1438) I. C. elegans Unc and Vul.
BW54 ct350 II. C. elegans Maintain at 15C. Temperature sensitive embryonic lethal. Sterile at 25C. Congenic strain, N2 and Bergerac BE. This allele should NOT be assumed to define a gene to which someone gave the name zyg-12. This name should not be used unless someone finds a non-complementing Bristol mutation. There is no evidence at present that the ts results from a single gene defect. Has been backcrossed >6 times to Bristol strains and should only contain Bergerac DNA in the unc-85 to dpy-10 region.
BW589 lin-10(e1438) unc-29(e1072) I. C. elegans Vul and Unc.
BW635 lin-10(e1438) daf-8(e1393) I. C. elegans Maintain at 15C. Vulvaless. Temperature-sensitive constitutive dauer (Daf-c) at 25C.
BW708 lin-28(n719) daf-8(e1393) I. C. elegans Egl. Constitutive dauer (Daf-c) at 25C.
BW774 lin-28(n719) unc-29(e1072) I. C. elegans Egl and Unc.
BW837 unc-29(e1072) lin-11(n566) I. C. elegans Vul and Unc.
BW983 bli-4(e937) unc-37(e262) I. C. elegans BliUnc. Blister seen in old adults.
CZ1072 unc-62(e917) V. C. elegans Inversion which may serve as a balancer for the center of LG V. Maternal effect lethal allele which results in 57% embryonic arrest, 40% larval arrest, and 3% which survive to be fertile adults with a variety of defects including Egl, Unc and Vab.
DH246 let-2(b246) X. C. elegans Temperature sensitive embryonic lethal. Grows at 15C, 20C. Lethal at 25C (embryonic). See also CGC 1806.
MH2003 lon-3(ct417) V. C. elegans Long.
MT1446 her-1(n695) V. C. elegans n695 XX animals are variably masculinized. Can be maintained as homozygous self-fertile hermaphrodite strain. n695 XO animals are WT males. n695 is weakly temperature sensitive: at 15C some XX animals are WT.
SB146 Caenorhabditis remanei ssp. remanei. C. remanei ssp. Rhabditis (Caenorhabditis) remanei ssp. remanei Sudhaus, 1974. Isolated in Freiburg, Germany from compost, associated with pill bugs. Likes to crawl off the plate and dig into the agar. Male/Female strain. Maintain by mating.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
ct102 Allele
ct60 Allele
ct169 Allele
ct171 Allele
ct174 Allele
ct176 Allele
ct177 Allele
ct181 Allele
ct223 Allele
ct351 Allele
ct226 Allele substitution
ct344 Allele deletion
ct41 Allele
ct49 Allele substitution nonsense
ct78 Allele
ct109 Allele
ct115 Allele
ct42 Allele substitution
ct45 Allele
ct46 Allele substitution
ct61 Allele
ct59 Allele
ct131 Allele
ct417 Allele substitution nonsense
ct73 Allele