Strain Information
Name | BFF69 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | mjIs134 II; hrde-1(pig6[degron::ha::hrde-1]) III; ieSi38 IV. |
Description | mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. N-terminal Auxin-Inducible Degron and HA tag inserted into the endogenous hrde-1 locus. Germline-expressed TIR1 and germline GFP. Allows for the degradation of hrde-1 and heritable RNAi deficiency in the presence of auxin. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343. |
Mutagen | |
Outcrossed | x0 |
Made by | Olga antonova |
Laboratory | BFF |
Reference | Toker, I. A., Lev, I., Mor, Y., Gurevich, Y., Fisher, D., Houri-Zeevi, L., ... & Rechavi, O. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv. |
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