Strain Information

Name BFF69   View On Wormbase
Species C. elegans
GenotypemjIs134 II; hrde-1(pig6[ha::degron::hrde-1]) III; ieSi38 IV.
DescriptionmjIs134 [mex-5p::GFP::(Gly)5Ala/his-58/tbb-2 3′UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. N-terminal HA-Degron tag inserted into the endogenous hrde-1 locus. Germline-expressed TIR1 and Germline GFP. Allows for the degradation of hrde-1 and heritable RNAi deficiency in the presence of auxin. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
MutagenNo mutagen
Outcrossedx0
Made byOlga antonova
Laboratory BFF
Reference Toker, I. A., Lev, I., Mor, Y., Gurevich, Y., Fisher, D., Houri-Zeevi, L., ... & Rechavi, O. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
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