Laboratory Information

NameBFF View on WormBase
Allele designationpig
HeadOded Rechavi
InstitutionTel Aviv University, Tel Aviv, Israel
Address Tel Aviv University
Ramat-Aviv,Haim Levanon st. P.O.B 39040
Neurobiology department
Tel Aviv 69978
Israel
Website http://www.odedrechavilab.com/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
BFF68 mjIs134 II; hrde-1(pig6[ha::degron::hrde-1]) III. C. elegans mjIs134 [mex-5p::GFP::(Gly)5Ala/his-58/tbb-2 3′UTR + Cbr-unc-119(+)] II. N-terminal HA-Degron tag inserted into the endogenous hrde-1 locus. gfp fluorescence in germline (mex-5::gfp). Control strain for BFF69; does not carry TIR1 transgene necessary for the degradation. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
BFF69 mjIs134 II; hrde-1(pig6[ha::degron::hrde-1]) III; ieSi38 IV. C. elegans mjIs134 [mex-5p::GFP::(Gly)5Ala/his-58/tbb-2 3′UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. N-terminal HA-Degron tag inserted into the endogenous hrde-1 locus. Germline-expressed TIR1 and Germline GFP. Allows for the degradation of hrde-1 and heritable RNAi deficiency in the presence of auxin. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
This laboratory hasn't submitted any alleles to the CGC.