Gene Information: hrde-1

Namehrde-1 View on WormBase
Species C. elegans
SequenceC16C10.3
Genetic positionIII:-3.71 +/- 0.003 cM
Genomic positionIII: 4172337..4176159

Strains carrying this gene

Strain Genotype Description
BFF68 mjIs134 II; hrde-1(pig6[ha::degron::hrde-1]) III. mjIs134 [mex-5p::GFP::(Gly)5Ala/his-58/tbb-2 3′UTR + Cbr-unc-119(+)] II. N-terminal HA-Degron tag inserted into the endogenous hrde-1 locus. gfp fluorescence in germline (mex-5::gfp). Control strain for BFF69; does not carry TIR1 transgene necessary for the degradation. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
BFF69 mjIs134 II; hrde-1(pig6[ha::degron::hrde-1]) III; ieSi38 IV. mjIs134 [mex-5p::GFP::(Gly)5Ala/his-58/tbb-2 3′UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. N-terminal HA-Degron tag inserted into the endogenous hrde-1 locus. Germline-expressed TIR1 and Germline GFP. Allows for the degradation of hrde-1 and heritable RNAi deficiency in the presence of auxin. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. (2020). Transgenerational Regulation of Sexual Attractiveness in C. elegans Nematodes. bioRxiv.
YY538 hrde-1(tm1200) III. Maintain at 15C. Heritable RNAi defective, germline mortality (Mrt) at 25C. Reference: Buckley BA, et al. Nature. 2012 Sep 20;489(7416):447-51.