| LP728 |
mig-5(cp385[mNG-GLO::AID::mig-5]) II. |
FP knock-in. mNG-GLO is a germline-optimized variant coded to be less prone to silencing in the germline. [NOTE: (4/1/2021) A lab has reported finding a second GFP insertion in LP728; it has not yet been confirmed whether or not it is present in the current CGC stock.] Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP705 |
dsh-1(cp375[dsh-1DIX::mNG-C1^3xFlag::PDZ,DEP]) II. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP435 |
apr-1(cp166[mNG-C1^3xFlag::apr-1]) I. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP713 |
pry-1(cp383[pry-1::mNG-C1^3xFlag]) I. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP530 |
cam-1(cp243[cam-1::mNG-C1^3xFlag]) II. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP527 |
mes-1(cp240[mes-1::mNG-C1^3xFlag] X. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP563 |
dnc-1(cp271[dnc::mNG-C1::3xFlag]) IV. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP585 |
lin-5(cp288[lin-5::mNG-C1^3xFlag]) II. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP560 |
dhc-1(cp268[dhc::mNG-C1::3xFlag]) I. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP439 |
nud-2(cp170[nud-2::mNG-C1^3xFlag]) I. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP451 |
bicd-1(cp180[mNG-C1^3xFlag::bicd-1]) IV. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP591 |
lis-1(cp294[lis-1::mNG-C1^3xFlag]) III. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP598 |
dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP447 |
klp-7(cp178[klp-7::mNG-C1^3xFlag]) III. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP443 |
klp-17(cp174[klp-17::mNG-C1^3xFlag]) II. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP521 |
klp-12(cp234[klp-12::mNG-C1^3xFlag]) IV. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP608 |
klp-20(cp311[klp-20::mNG-C1^3xFlag]) III. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP600 |
klp-4(cp303[klp-4::mNG-C1^3xFlag]) X. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| LP604 |
klp-8(cp307[klp-8::mNG-C1^3xFlag]) X. |
FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press. |
| SWF7 |
flvEx5. |
flvEx5 [rig-3p::wArchon1::GFP + elt-2p::nGFP]. Pick GFP+ to maintain. Expresses voltage sensor wArchon1 in AVA, tagged with GFP, as well as GFP in the gut from the co-injection marker. |
| OW716 |
tdo-2(zg217) III. |
Crispr/Cas9 engineered deletion mutant removes 14 basepairs in tdo-2 coding region. Reference: Michels H, et al. Sci Rep. 2016 Dec 20;6:39199. |
| OW717 |
tdo-2(zg218) III. |
Crispr/Cas9 engineered deletion mutant removes 9 basepairs in tdo-2 coding region. Reference: Michels H, et al. Sci Rep. 2016 Dec 20;6:39199. |
| OW454 |
kynu-1(tm4924) X. |
C15H9.7. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7. |
| OW477 |
afmd-1(tm4547) IV. |
D2024.2. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7. |
| OW478 |
kmo-1(tm4529) V. |
R07B7.5. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7. |
| OW479 |
haao-1(tm4627) V. |
K06A4.5. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7. |
| OW452 |
moag-4(tm4909) I. |
Homozygous viable. Reference: van Ham TJ, et al. Cell. 2010 Aug 20;142(4):601-12. |
| OW1002 |
lir-3(tm813) II. |
Homozygous viable. Reference: Sin O, et al. Mol Cell. 2017 Mar 16;65(6):1096-1108. |
| XE1220 |
wpEx76. |
wpEx76 [myo-3p::tom20::tdKillerRed + unc-122p::GFP]. Pick GFP+ to maintain. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpEx76 carries a tandem dimer version of KillerRed targeted to the mitochondria by the addition of a tom-20 targeting sequence (mito-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63. |
| XE1142 |
wpIs14 X. |
wpIs14 [unc-17p::KillerRed + unc-122p::GFP] X. X-Linked based on out-cross data. KillerRed expression in acetylcholine neurons. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63. |
| XE1550 |
wpIs59. |
wpIs59 [unc-47p::MYR::tdKillerRed]. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs59 carries a tandem dimer version of KillerRed targeted to the plasma membrane through the addition of a myristoylation tag (mry-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63. |
| MT15883 |
csp-2(n4871) IV. |
n4871 is a 1136 bp deletion that removes the last five exons, including the putative protease active site. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341. |
| MT15884 |
csp-3(n4872) I. |
n4872 is a 722 bp deletion that removes part of exon 2 and all of exons 3 and 4. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341. |
| MT16846 |
csp-1(n4967) II. |
n4967 is a 769 bp deletion that removes the putative protease active site. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341. |
| VV213 |
Y53F4B.18(vq3) II. |
Superficially wild-type. Reference: Chen AL, et al. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0243-4. |
| OD3609 |
faah-4(lt121) III. |
NOTE: This strain can be sensitive to starvation; the authors recommend that anyone using this strain for their work should propagate the stock for several generations after starvation before conducting experiments. Reference: Chen AL, et al. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0243-4. |
| EU1303 |
cyk-4(or570) III. |
Maintain at 15C. Temperature-sensitive embryonic lethal with cytokinesis defects. Reference: Canman JC, et al. Science. 2008 Dec 5;322(5907):1543-6. |
| MT22914 |
gcn-1(n4827) III. |
n4827 is a null allele of gcn-1 and that loss of gcn-1 function causes a defect in M4 sister cell death. Reference: Hirose T & Horvitz HR. PLoS Genet. 2014 Aug 7;10(8):e1004512. |
| GR3090 |
mgIs77 V. |
mgIs77 [rpl-28p::ub(G76V)::GFP + unc-119(+) + myo-2p::mCherry] V. Reference: Lehrbach NJ, et al. Cell. 2019 Apr 18;177(3):737-750.e15. |
| GR3149 |
mgIs72 II; mgIs78 IV. |
mgIs72 [rpt-3p::GFP + dpy-5(+)] II. mgIs78 [myo-3p::H2B::mCherry::SL2::pbs-5(T65A)] IV. Muscle-specific proteasome dysfunction. Reference: Lehrbach NJ & Ruvkun G. Elife. 2019 Apr 11;8. pii: e44425. doi: 10.7554/eLife.44425. |
| AT18 |
srf-6(yj13) II. |
No visible phenotype. Whole genome sequenced strain. Reference: Grenache DG, et al. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12388-93. |
| AT25 |
srf-6(yj41) II. |
No visible phenotype. Whole genome sequenced strain. |
| EU3206 |
sas-6(or1167) IV. |
Temperature-sensitive embryonic lethal. Maintain at 15C with 20% to 50% hatching rate. 100% lethality and mono-polar spindle at 26C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. |
| CZ27508 |
micu-1(ju1783[micu-1::gfp]) IV. |
GFP reporter inserted into C-terminus of endogenous micu-1 locus. Diffuse GFP signals throughout the body. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061. |
| CZ26660 |
micu-1(ju1155) IV/nT1 [qIs51] (IV;V). |
Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP micu-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061. |
| AVS411 |
artEx30. |
artEx30 [hpk-1p::hpk-1::tdTomato + hsf-1p::hsf-1::GFP + rol-6 (su1006)]. Pick Rollers to maintain. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. |
| AVS397 |
gpIs1; artEx35. |
gpIs1 [hsp-16.2p::GFP]. artEx35 [sur-5p::hpk-1::CFP + myo-2p::mCherry)]. Pick animals with red pharynx to maintain. Inducible GFP fluorescence after >1 hour heat shock. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. |
| EU3115 |
klp-15(ok1958) klp-16(or1952)/tmC18[dpy-5(tmIs1236)] I; ltIs37 IV; ruIs57. |
ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry klp-15/16 homozygotes. Homozygous double deletion mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 |
| EU3201 |
klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; ltIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV |
ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 |
| OG1110 |
ogt-1(dr20) III; drIs4 IV; drEx468. |
drIs4 [gpdh-1p::GFP + col-12p::DsRed] IV. drEx468 [ogt-1p::ogt-1(cDNA)::ogt-1 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Over-expression of OGT-1 in presumptive null mutant ogt-1(dr20) background. gpdh-1p::GFP is induced during hypertonic stress. Constitutive col-12p::DsRed expression. Reference: Urso SJ, et al. (2020). The O-GlcNAc transferase OGT is a conserved and essential regulator of the cellular and organismal response to hypertonic stress. bioRxiv, 2020.2005.2001.072033. |