Species Information: C elegans

Name C elegans
NCBI Taxonomy ID

C elegans strains available at the CGC

Strain Genotype Description
LP728 mig-5(cp385[mNG-GLO^AID::mig-5]) II. FP knock-in. [NOTE: (4/1/2021) A lab has reported finding a second GFP insertion in LP728; it has not yet been confirmed whether or not it is present in the current CGC stock.] Reference: Heppert JK, et al. 2017 Genetics. In press.
LP705 dsh-1(cp375[dsh-1DIX::mNG-C1^3xFlag::PDZ,DEP]) II. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP435 apr-1(cp166[mNG-C1^3xFlag::apr-1]) I. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP713 pry-1(cp383[pry-1::mNG-C1^3xFlag]) I. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP530 cam-1(cp243[cam-1::mNG-C1^3xFlag]) II. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP527 mes-1(cp240[mes-1::mNG-C1^3xFlag] X. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP563 dnc-1(cp271[dnc::mNG-C1::3xFlag]) IV. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP585 lin-5(cp288[lin-5::mNG-C1^3xFlag]) II. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP560 dhc-1(cp268[dhc::mNG-C1::3xFlag]) I. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP439 nud-2(cp170[nud-2::mNG-C1^3xFlag]) I. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP451 bicd-1(cp180[mNG-C1^3xFlag::bicd-1]) IV. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP591 lis-1(cp294[lis-1::mNG-C1^3xFlag]) III. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP598 dlg-1(cp301[dlg-1::mNG-C1^3xFlag]) X. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP447 klp-7(cp178[klp-7::mNG-C1^3xFlag]) III. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP443 klp-17(cp174[klp-17::mNG-C1^3xFlag]) II. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP521 klp-12(cp234[klp-12::mNG-C1^3xFlag]) IV. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP608 klp-20(cp311[klp-20::mNG-C1^3xFlag]) III. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP600 klp-4(cp303[klp-4::mNG-C1^3xFlag]) X. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
LP604 klp-8(cp307[klp-8::mNG-C1^3xFlag]) X. FP knock-in. Reference: Heppert JK, et al. 2017 Genetics. In press.
SWF7 flvEx5. flvEx5 [rig-3p::wArchon1::GFP + elt-2p::nGFP]. Pick GFP+ to maintain. Expresses voltage sensor wArchon1 in AVA, tagged with GFP, as well as GFP in the gut from the co-injection marker.
OW716 tdo-2(zg217) III. Crispr/Cas9 engineered deletion mutant removes 14 basepairs in tdo-2 coding region. Reference: Michels H, et al. Sci Rep. 2016 Dec 20;6:39199.
OW717 tdo-2(zg218) III. Crispr/Cas9 engineered deletion mutant removes 9 basepairs in tdo-2 coding region. Reference: Michels H, et al. Sci Rep. 2016 Dec 20;6:39199.
OW454 kynu-1(tm4924) X. C15H9.7. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7.
OW477 afmd-1(tm4547) IV. D2024.2. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7.
OW478 kmo-1(tm4529) V. R07B7.5. Homozygous viable. Reference: van der Goot AT, et al. Proc. Natl. Acad. Sci. U.S.A. 2012 109(37) 14912-7.
OW452 moag-4(tm4909) I. Homozygous viable. Reference: van Ham TJ, et al. Cell. 2010 Aug 20;142(4):601-12.
OW1002 lir-3(tm813) II. Homozygous viable. Reference: Sin O, et al. Mol Cell. 2017 Mar 16;65(6):1096-1108.
XE1220 wpEx76. wpEx76 [myo-3p::tom20::tdKillerRed + unc-122p::GFP]. Pick GFP+ to maintain. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpEx76 carries a tandem dimer version of KillerRed targeted to the mitochondria by the addition of a tom-20 targeting sequence (mito-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1142 wpIs14 X. wpIs14 [unc-17p::KillerRed + unc-122p::GFP] X. X-Linked based on out-cross data. KillerRed expression in acetylcholine neurons. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1550 wpIs59. wpIs59 [unc-47p::MYR::tdKillerRed]. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs59 carries a tandem dimer version of KillerRed targeted to the plasma membrane through the addition of a myristoylation tag (mry-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
MT15883 csp-2(n4871) IV. n4871 is a 1136 bp deletion that removes the last five exons, including the putative protease active site. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341.
MT15884 csp-3(n4872) I. n4872 is a 722 bp deletion that removes part of exon 2 and all of exons 3 and 4. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341.
MT16846 csp-1(n4967) II. n4967 is a 769 bp deletion that removes the putative protease active site. Reference: Denning DP, et al. PLoS Genet. 2013;9(3):e1003341.
VV213 Y53F4B.18(vq3) II. Superficially wild-type. Reference: Chen AL, et al. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0243-4.
OD3609 faah-4(lt121) III. NOTE: This strain can be sensitive to starvation; the authors recommend that anyone using this strain for their work should propagate the stock for several generations after starvation before conducting experiments. Reference: Chen AL, et al. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0243-4.
EU1303 cyk-4(or570) III. Maintain at 15C. Temperature-sensitive embryonic lethal with cytokinesis defects. Reference: Canman JC, et al. Science. 2008 Dec 5;322(5907):1543-6.
MT22914 gcn-1(n4827) III. n4827 is a null allele of gcn-1 and that loss of gcn-1 function causes a defect in M4 sister cell death. Reference: Hirose T & Horvitz HR. PLoS Genet. 2014 Aug 7;10(8):e1004512.
GR3090 mgIs77 V. mgIs77 [rpl-28p::ub(G76V)::GFP + unc-119(+) + myo-2p::mCherry] V. Reference: Lehrbach NJ, et al. Cell. 2019 Apr 18;177(3):737-750.e15.
GR3149 mgIs72 II; mgIs78 IV. mgIs72 [rpt-3p::GFP + dpy-5(+)] II. mgIs78 [myo-3p::H2B::mCherry::SL2::pbs-5(T65A)] IV. Muscle-specific proteasome dysfunction. Reference: Lehrbach NJ & Ruvkun G. Elife. 2019 Apr 11;8. pii: e44425. doi: 10.7554/eLife.44425.
AT18 srf-6(yj13) II. No visible phenotype. Whole genome sequenced strain. Reference: Grenache DG, et al. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12388-93.
AT25 srf-6(yj41) II. No visible phenotype. Whole genome sequenced strain.
EU3206 sas-6(or1167) IV. Temperature-sensitive embryonic lethal. Maintain at 15C with 20% to 50% hatching rate. 100% lethality and mono-polar spindle at 26C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644.
CZ27508 micu-1(ju1783[micu-1::gfp]) IV. GFP reporter inserted into C-terminus of endogenous micu-1 locus. Diffuse GFP signals throughout the body. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061.
CZ26660 micu-1(ju1155) IV/nT1 [qIs51] (IV;V). Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP micu-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061.
AVS411 artEx30. artEx30 [hpk-1p::hpk-1::tdTomato + hsf-1p::hsf-1::GFP + rol-6 (su1006)]. Pick Rollers to maintain. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188.
AVS397 gpIs1; artEx35. gpIs1 [hsp-16.2p::GFP]. artEx35 [sur-5p::hpk-1::CFP + myo-2p::mCherry)]. Pick animals with red pharynx to maintain. Inducible GFP fluorescence after >1 hour heat shock. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188.
EU3115 klp-15(ok1958) klp-16(or1952)/tmC18[dpy-5(tmIs1236)] I; itIs37 IV; ruIs57. itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry klp-15/16 homozygotes. Homozygous double deletion mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020
EU3201 klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; itIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020
OG1110 ogt-1(dr20) III; drIs4 IV; drEx468. drIs4 [gpdh-1p::GFP + col-12p::DsRed] IV. drEx468 [ogt-1p::ogt-1(cDNA)::ogt-1 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Over-expression of OGT-1 in presumptive null mutant ogt-1(dr20) background. gpdh-1p::GFP is induced during hypertonic stress. Constitutive col-12p::DsRed expression. Reference: Urso SJ, et al. (2020). The O-GlcNAc transferase OGT is a conserved and essential regulator of the cellular and organismal response to hypertonic stress. bioRxiv, 2020.2005.2001.072033.
EU3030 ijmSi3 I; unc-119(ed3) III; itIs37 IV. ijmSi3 [mex-5p::cls-2(re-encoded)::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] I. itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. The coding sequence of GFP-tagged cls-2 in the transgene was re-coded using silent mutations to render it insensitive to RNAi-depletion of endogenous cls-2 expression. mCherry expression marks histones. Not known if unc-119(ed3) is still carried in the background of this strain. Reference: Schlientz A and Bowerman B. PLoS Genet. 2020 Oct 7;16(10):e1008751. doi: 10.1371/journal.pgen.1008751.