Laboratory Information
Name | TQ View on WormBase |
---|---|
Allele designation | xu |
Head | Shawn Xu |
Institution | University of Michigan, Ann Arbor, MI |
Address | Life Sciences Institute, Rm 6239 University of Michigan 210 Washtenaw Ave. Ann Arbor 48109 United States |
Website | http://www.lsi.umich.edu/labs/shawn-xu-lab |
Gene classes |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
OP50(xu363) | E. coli [ura-, strR, rnc-, (delta)attB::FRT-lacI-lacUV5p-T7)]. | Escherichia coli | Bacteria. RNAi-compatible OP50 strain. Slow growing. When growing this strain, pick single colonies for liquid culture (at least 20 hrs) from a freshly streaked tetracycline LB plate. Do not include tetracycline in liquid culture, as Tet in liquid culture can have long lasting effects on worm lifespan. The authors recommend using standard PEG transformation method to make competent OP50(xu363) cells, but other ways to make competent cells will also likely work for OP50(xu363). Reference: Xiao R, et al. Cell Rep. 2015 May 19;11(7):1123-33. |
OP50-tdTomato | E. coli | Escherichia coli | Bacteria. A22 tdTomato-expressing OP50. Amp Resistant. tdTomato coding sequence was cloned into pGEX-5x-3 vector and transformed into OP50 component cells. Reference: Zhang B, et al. Nat Aging 2021 1, 255–268. https://doi.org/10.1038/s43587-021-00039-1. |
TQ10515 | seld-1(xu408) IV. | C. elegans | seld-1(xu408) is a G314E missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10516 | scbp-2(xu418) I. | C. elegans | scbp-2(xu418) is a G47R missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10517 | gspd-1(xu416) IV. | C. elegans | gspd-1(xu416) is a E375K missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10518 | gspd-1(xu409) IV. | C. elegans | gspd-1(xu409) is a L369F missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10519 | gsr-1(xu413) III. | C. elegans | gsr-1(xu413) is a S21F missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10520 | seld-1(xu415) IV. | C. elegans | seld-1(xu415) is a G170E missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10521 | gsr-1(xu414) III. | C. elegans | gsr-1(xu414) is a G279E missense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ10548 | trxr-1(xu421) IV. | C. elegans | trxr-1(xu421) is a W275* nonsense mutation; suppresses LITE-1 function. Reference: Zhang W, et al. PLOS Genetics 2020 Dec 10;16(12):e1009257. PMID: 33301443 |
TQ1101 | lite-1(xu7) X. | C. elegans | Defective phototaxis (light avoidance). To identify lite-1(xu7) homozygotes, place day 1 adults on a freshly seeded NGM plate with a thin lawn of OP50. Deliver 2 second pulses of short wavelength light (UV, purple, blue) from an arc lamp to the head of a worm that is slowly moving forward through a 5-10x objective lens in conjunction with a room lens under a fluorescent dissection scope. Manually move the plate so only the anterior of the worm appears in the field of view. Wild-type worms respond by initiating reversals while homozygous mutants do not. Maintain under normal conditions. Reference: Liu J, et al (2010) Nature Neurosci 13:715-22. |
TQ1828 | pde-1(nj57) pde-5(nj49) I; pde-3(nj59) II; pde-2(tm3098) III. | C. elegans | Maintain under normal conditions. Reference: Liu J, et al (2010) Nature Neurosci 13:715-22. |
TQ194 | trp-2(sy691) III. | C. elegans | |
TQ2183 | lite-1(xu7) X; xuEx705. | C. elegans | xuEx705 [npr-9p::GCaMP3.0 + npr-9::DsRed2B]. Superficially wild-type. Maintain by picking red fluorescent animals; DsRed might not be visible at lower magnifications. Reference: Piggott BJ, et al. Cell. 2011 Nov 11;147(4):922-33. |
TQ225 | trp-1(sy690) III. | C. elegans | |
TQ233 | trpa-1(ok999) IV. | C. elegans | Shorter lifespan than wild-type worms at 15-20 C, but not at 25 C. Reference: Xiao R, et al. Cell. 2013 Feb 14;152(4):806-17. |
TQ296 | trp-4(sy695) I. | C. elegans | |
TQ3032 | lite-1(xu7) X; xuEx1040. | C. elegans | xuEx1040 [nmr-1p::G-CaMP3.0 + nmr-1p::DsRed]. Pick fluorescent animals to maintain. Reference: Piggott BJ, et al. Cell. 2011 Nov 11;147(4):922-33. |
TQ8245 | lite-1(xu492) X. | C.elegans | Light sensation defect; loss of light sensation. lite-1(xu492) is a 2701 bp deletion generated by CRISPR/Cas9-based gene editing using the Fire Lab protocol (Arribere et al., 2014). Left flanking sequence: 5’ CGTAAAAAACAACATGCCACCAC Right flanking sequence: 5' GGCGGCCACCTACGCCAGTA. Primer sequences used to detect the deletion: Forward (flanking): 5’ GAAGAAAAGGCGGTGCAAAC; Reverse (flanking): 5’ GAAGCAACAAGACGATCTCC; Forward (internal): 5’ ATGATCGCAAAAATCCTGTCGAGTC. Wild-type product: 1972 bp; xu492 product: 1475 bp; both bands should be visible if heterozygous. Reference: Zhang W, et al. PLoS Genet. 2020 Dec 10;16(12):e1009257. doi: 10.1371/journal.pgen.1009257. eCollection 2020 Dec. |
Alleles contributed by this laboratory
Allele | Type | DNA Change | Protein Change |
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xu7 | Allele | substitution |