Laboratory Information
| Name | HS View on WormBase |
|---|---|
| Allele designation | os |
| Head | Hitoshi Sawa |
| Institution | National Institute of Genetics, Mishima, Japan |
| Address | Multicellular Organization Laboratory National Institute of Genetics 1111　Yata Mishima 411-8540 Japan |
| Website | http://www.nig.ac.jp/labs/MultiOrg/Multicellular/index-e.html |
| Gene classes | bet psa rmd pign |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| HS1028 | mom-4(ne1539) I; lit-1(t1512) III. | C. elegans | This strain can grow at 11.5C but is embryonic lethal at 15C and above. Temperature shifts in late embryogenesis or in larval stages result in defects in many asymmetric cell divisions in postembryonic develoment. |
| HS1204 | rmd-1&T05G5.9(tm1457) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are green and segregate green WT, dead eggs and nonGreens that lay dead eggs with the defects in spindle organization, chromosome segregation, and cytokinesis. |
| HS1215 | unc-76(e911) V; osEx211. | C. elegans | osEx211[apr-1::GFP + unc-76(+)]. This strain expresses functional APR-1::GFP driven by the apr-1 promoter. In the seam cells, just prior to the onset of mitosis, APR-1::GFP localizes to the anterior cortex. |
| HS1222 | pbrm-1(tm415) I. | C. elegans | Sys. Low penetrance Psa. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. |
| HS1257 | unc-76(e911) V; osEx219. | C. elegans | osEx219 [pbrm-1::GFP + unc-76(+)]. Pick wild-type to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. |
| HS1294 | unc-76(e911) V; osEx225. | C. elegans | osEx225 [scm::dsh-2::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. |
| HS1325 | unc-76(e911) V; osEx229. | C. elegans | osEx229 [pry-1::GFP + unc-76(+)]. This strain expresses functional pry-1::GFP driven by the pry-1 promoter. In the seam cells, just prior to the onset of mitosis, pry-1::GFP localizes to the anterior cortex. |
| HS1337 | osIs1. | C. elegans | osIs1 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG II. This strain has Ste, Emb, Muv, Him phenotypes (probably dominant effects of the integration). Expression in many blast cells can be detected. Reference: Fujita et al. PLoS ONE 2, e407 (2007). |
| HS1339 | osIs2. | C. elegans | osIs2 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG X. No dominant phenotypes observed (see HS1337). Expression in many blast cells can be detected, but much weaker than osIs1. Reference: Fujita et al. PLoS ONE 2, e407 (2007). |
| HS1359 | unc-76(e911) V; osEx233. | C. elegans | osEx233 [scm::mig-5::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. |
| HS1380 | unc-76(e911) V; osEx240. | C. elegans | osEx240 [bet-1p::bet-1::GFP + unc-76(+)]. Pick Wild-type (non-Unc) to maintain. GFP expression in most somatic cells. Reference: Shibata Y, et al. Development. 2010 Apr;137(7):1045-53. |
| HS1417 | osIs5 II. | C. elegans | osIs5 [scm::wrm-1::Venus + unc-76(+)]. WRM-1::GFP localizes to the anterior cortex in the seam cells prior to or during cell division, and to the posterior daughter's nucleus after cell division. |
| HS143 | msi-1(os1) III. | C. elegans | 1.6 kb deletion in msi-1. Males have poor mating efficiency. |
| HS144 | msi-1(os1) III; him-5(e1490) V. | C. elegans | 1.6 kb deletion in msi-1. Males have poor mating efficiency. |
| HS1673 | lin-17(n3091) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); vpIs1 X. | C. elegans | vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP n3091 homozygotes (Sys Unc Psa). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS1675 | egl-20(n585) cwn-2(ok895) IV. | C. elegans | Egl. Unc. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS1680 | lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. | C. elegans | zdIs5 [mec-4p::GFP + lin-15(+)] I. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C. |
| HS169 | nob-1(os6) III. | C. elegans | Tail abnormal. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype. |
| HS1698 | unc-119(ed3) III; osIs15. | C. elegans | osIs15 [pie-1p::GFP::apr-1 + unc-119(+)]. Non-Unc. Reference: Cell. 2011 Sep 16;146(6):942-54. |
| HS1749 | mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS178 | psa-3(os8) X. | C. elegans | Superficially WT. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype. |
| HS1790 | mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) V. | C. elegans | mig-1 confirmed by complementation tests, and cfz-2 by PCR. Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS1795 | dsh-2(or302) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) mig-5(tm2639) homozygotes (Sys). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. |
| HS184 | swsn-4(os13) IV. | C. elegans | Egl, Pvul, Psa (Phasmid Socket Absent) and some embryonic lethality. The T cell division can be symmetric as in lin-17 mutants. Less severe at 15C. swsn-4 encodes a homolog of yeast SW12, a component of the SWI/SNF complex. |
| HS2067 | mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) wIs51 V; lin-18(e620) X. | C. elegans | wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Heterozygotes are GFP+(pharynx) wild-type and segregate GFP+(pharynx) wild-type, GFP-(pharynx) Sys Psa Unc and dead eggs. PIck GFP+(pharynx) wild-type to maintain. Presence of cfz-2 was confirmed by PCR; mig-1 by complementation test. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2326 | cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); vpIs1 X. | C. elegans | vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok546 homozygotes (Unc Egl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2329 | unc-76(e911) V; osEx397. | C. elegans | osEx397 [cwn-1p::cwn-1::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in tail region. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2332 | unc-76(e911) V; osEx393. | C. elegans | osEx393 [cwn-2p::cwn-2::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in the pharynx. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2372 | mig-1(e1787) I; cfz-2(ok1201) wIs51 V; lin-18(e620) X. | C. elegans | wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Bivulva. Presence of cfz-2 was confirmed by PCR. mig-1 and lin-18 were confirmed by sequencing. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. |
| HS2690 | dsh-2(or302) dsh-1(ok1445)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) dsh-1(ok1445) homozygotes (Sys Unc). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. |
| HS2725 | dsh-2(or302) dsh-1(ok1445) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and non-GFP triple homozygotes (mostly Emb with a few animals surviving to early L1). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. |
| HS2728 | dsh-1(ok1445) mig-5(tm2639) II. | C. elegans | One or no gonad arm. Frequently ruptures at vulva. |
| HS304 | swsn-1(os22) V. | C. elegans | Temperature sensitive. At 22.5C, maternal effect embryonic lethal. Temperature shift-up to 22.5C during embryogenesis results in animals with Egl, Pvul and Psa (phasmid socket absent) phenotypes. Shift-up to 25C results in growth arrest at larval stage. The T cell division can be symmetric as in lin-17 mutants. At 15C, nearly WT. Males grown at 15C can mate very well. psa-1 encodes a homolog of yeast SW13, a component of the SWI/SNF complex. Sequence data of this strain revealed the mutation is actually GTC/CCC/TCA to GTC/CTC/TCA causing a P86L substitution (G. Hayes). |
| HS321 | him-5(e1467) unc-76(e911) V; osEx67. | C. elegans | osEx67 [psa-4::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. |
| HS325 | him-5(e1467) unc-76(e911) V; osEx71. | C. elegans | osEx71 [psa-1::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. |
| HS3528 | osIs158 II. | C. elegans | osIs158 [eft-3p::AtTIR1(F79G)::mRuby] II. Single copy insertion into ttTi5605 on LG II. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. |
| HS3545 | osIs158 II; ieSi58 IV. | C. elegans | osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. |
| HS3750 | ieSi58 IV; osIs182 V. | C. elegans | ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. osIs182 [eft-3p::AtTIR1(F79G) + LoxP + myo-2p::GFP + rps-27p::neoR + LoxP] V. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. osIs182 is a single copy insertion of TIR1(F79G) into chromosome V (oxTi365) and expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. |
| HS399 | let-526(os37) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT and GFP+. os37 homozygotes are Lvl and Psa. Pick GFP+ heterozygotes to maintain. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. |
| HS411 | ceh-20(os39) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT and GFP+. Segregate GFP- sterile Unc Psa (phasmid socket absent), very rare homozygous hT2 glowing animals, and dead eggs. ceh-20(os39) animals show defects in asymmetric T cell division. |
| HS428 | dpy-22(os26) X; osEx89. | C. elegans | osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-. |
| HS445 | dpy-22(os38) X; osEx89. | C. elegans | osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-. |
| HS458 | let-19(os33)/mIn1 [dpy-10(e128) mIs14] II. | C. elegans | Heterozygotes are WT GFP+ and segregate WT GFP+, Dpy GFP+ (mIn1 homozygotes), and os33 homozygotes (GFP-, Dpy, Muv, Steriles). |
| HS616 | osEx108. | C. elegans | osEx108 [(pAY105) let-19::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. Reference: Yoda et al. Development (2005) vol. 132 (8) pp. 1885-93. |
| HS634 | rnt-1(os58) I. | C. elegans | Phasmid socket absent. |
| HS661 | nob-1(os68) III. | C. elegans | Healthy. Abnormal morphology of the tail (only at Nomarski level). Defects in asymmetric T cell division causes Psa (phasmid socket absent). |
| HS732 | wrm-1(tm514) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm514 homozygotes (probable embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. |
| HS845 | osEx138. | C. elegans | osEx138 [let-526::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. |
| HS886 | bet-1(os46) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). | C. elegans | Heterozygotes are WT and GFP in the pharynx, and segregate WT GFP+, Sterile Psa(Phasmid socket absent) non-GFP homozygous os46) animals and dead eggs. Maintain by picking GFP progeny. Reference: Shibata et al. Development in press (2010). |
| HS942 | unc-76(e911) V; osEx158. | C. elegans | osEx158 [wrm-1::GFP + unc-76(+)]. Animals with the array are WT and GFP+. Animals which have lost the array are Unc and GFP-. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| os1 | Allele | deletion | |
| os6 | Allele | ||
| os8 | Allele | deletion | |
| os13 | Allele | substitution | |
| os22 | Allele | substitution | |
| os37 | Allele | ||
| os39 | Allele | ||
| os26 | Allele | substitution | |
| os38 | Allele | substitution | |
| os33 | Allele | substitution | nonsense |
| os58 | Allele | substitution | nonsense |
| os68 | Allele | ||
| os46 | Allele | substitution | nonsense |