Laboratory Information

NameHS View on WormBase
Allele designationos
HeadHitoshi Sawa
InstitutionNational Institute of Genetics, Mishima, Japan
Address Multicellular Organization Laboratory
National Institute of Genetics
1111 Yata
Mishima 411-8540
Japan
Website http://www.nig.ac.jp/labs/MultiOrg/Multicellular/index-e.html
Gene classes bet  psa  rmd  pign 

Strains contributed by this laboratory

Strain Genotype Species Description
HS1028 mom-4(ne1539) I; lit-1(t1512) III. C. elegans This strain can grow at 11.5C but is embryonic lethal at 15C and above. Temperature shifts in late embryogenesis or in larval stages result in defects in many asymmetric cell divisions in postembryonic develoment.
HS1204 rmd-1&T05G5.9(tm1457) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are green and segregate green WT, dead eggs and nonGreens that lay dead eggs with the defects in spindle organization, chromosome segregation, and cytokinesis.
HS1215 unc-76(e911) V; osEx211. C. elegans osEx211[apr-1::GFP + unc-76(+)]. This strain expresses functional APR-1::GFP driven by the apr-1 promoter. In the seam cells, just prior to the onset of mitosis, APR-1::GFP localizes to the anterior cortex.
HS1222 pbrm-1(tm415) I. C. elegans Sys. Low penetrance Psa. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57.
HS1257 unc-76(e911) V; osEx219. C. elegans osEx219 [pbrm-1::GFP + unc-76(+)]. Pick wild-type to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57.
HS1294 unc-76(e911) V; osEx225. C. elegans osEx225 [scm::dsh-2::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99.
HS1325 unc-76(e911) V; osEx229. C. elegans osEx229 [pry-1::GFP + unc-76(+)]. This strain expresses functional pry-1::GFP driven by the pry-1 promoter. In the seam cells, just prior to the onset of mitosis, pry-1::GFP localizes to the anterior cortex.
HS1337 osIs1. C. elegans osIs1 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG II. This strain has Ste, Emb, Muv, Him phenotypes (probably dominant effects of the integration). Expression in many blast cells can be detected. Reference: Fujita et al. PLoS ONE 2, e407 (2007).
HS1339 osIs2. C. elegans osIs2 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG X. No dominant phenotypes observed (see HS1337). Expression in many blast cells can be detected, but much weaker than osIs1. Reference: Fujita et al. PLoS ONE 2, e407 (2007).
HS1359 unc-76(e911) V; osEx233. C. elegans osEx233 [scm::mig-5::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99.
HS1380 unc-76(e911) V; osEx240. C. elegans osEx240 [bet-1p::bet-1::GFP + unc-76(+)]. Pick Wild-type (non-Unc) to maintain. GFP expression in most somatic cells. Reference: Shibata Y, et al. Development. 2010 Apr;137(7):1045-53.
HS1417 osIs5 II. C. elegans osIs5 [scm::wrm-1::Venus + unc-76(+)]. WRM-1::GFP localizes to the anterior cortex in the seam cells prior to or during cell division, and to the posterior daughter's nucleus after cell division.
HS143 msi-1(os1) III. C. elegans 1.6 kb deletion in msi-1. Males have poor mating efficiency.
HS144 msi-1(os1) III; him-5(e1490) V. C. elegans 1.6 kb deletion in msi-1. Males have poor mating efficiency.
HS1673 lin-17(n3091) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); vpIs1 X. C. elegans vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP n3091 homozygotes (Sys Unc Psa). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1675 egl-20(n585) cwn-2(ok895) IV. C. elegans Egl. Unc. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1680 lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. C. elegans zdIs5 [mec-4::GFP + lin-15(+)]. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C.
HS169 nob-1(os6) III. C. elegans Tail abnormal. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype.
HS1698 unc-119(ed3) III; osIs15. C. elegans osIs15 [pie-1p::GFP::apr-1 + unc-119(+)]. Non-Unc. Reference: Cell. 2011 Sep 16;146(6):942-54.
HS1749 mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS178 psa-3(os8) X. C. elegans Superficially WT. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype.
HS1790 mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) V. C. elegans mig-1 confirmed by complementation tests, and cfz-2 by PCR. Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS1795 dsh-2(or302) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) mig-5(tm2639) homozygotes (Sys). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
HS184 swsn-4(os13) IV. C. elegans Egl, Pvul, Psa (Phasmid Socket Absent) and some embryonic lethality. The T cell division can be symmetric as in lin-17 mutants. Less severe at 15C. swsn-4 encodes a homolog of yeast SW12, a component of the SWI/SNF complex.
HS2067 mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) wIs51 V; lin-18(e620) X. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Heterozygotes are GFP+(pharynx) wild-type and segregate GFP+(pharynx) wild-type, GFP-(pharynx) Sys Psa Unc and dead eggs. PIck GFP+(pharynx) wild-type to maintain. Presence of cfz-2 was confirmed by PCR; mig-1 by complementation test. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS2326 cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); vpIs1 X. C. elegans vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok546 homozygotes (Unc Egl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS2329 unc-76(e911) V; osEx397. C. elegans osEx397 [cwn-1p::cwn-1::Venus] Transgene is expressed in tail region. Pick nonUnc to maintain. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS2332 unc-76(e911) V; osEx393. C. elegans osEx393 [cwn-2p::cwn-2::Venus] Transgene is expressed in the pharynx. Pick nonUnc to maintain. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS2372 mig-1(e1787) I; cfz-2(ok1201) wIs51 V; lin-18(e620) X. C. elegans wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Bivulva. Presence of cfz-2 was confirmed by PCR. mig-1 and lin-18 were confirmed by sequencing. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308.
HS2690 dsh-2(or302) dsh-1(ok1445)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) dsh-1(ok1445) homozygotes (Sys Unc). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
HS2725 dsh-2(or302) dsh-1(ok1445) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and non-GFP triple homozygotes (mostly Emb with a few animals surviving to early L1). Pick WT GFP+ animals and check for correct segregation of progeny to maintain.
HS2728 dsh-1(ok1445) mig-5(tm2639) II. C. elegans One or no gonad arm. Frequently ruptures at vulva.
HS304 swsn-1(os22) V. C. elegans Temperature sensitive. At 22.5C, maternal effect embryonic lethal. Temperature shift-up to 22.5C during embryogenesis results in animals with Egl, Pvul and Psa (phasmid socket absent) phenotypes. Shift-up to 25C results in growth arrest at larval stage. The T cell division can be symmetric as in lin-17 mutants. At 15C, nearly WT. Males grown at 15C can mate very well. psa-1 encodes a homolog of yeast SW13, a component of the SWI/SNF complex. Sequence data of this strain revealed the mutation is actually GTC/CCC/TCA to GTC/CTC/TCA causing a P86L substitution (G. Hayes).
HS321 him-5(e1467) unc-76(e911) V; osEx67. C. elegans osEx67 [psa-4::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24.
HS325 him-5(e1467) unc-76(e911) V; osEx71. C. elegans osEx71 [psa-1::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24.
HS3528 osIs158 II. C. elegans osIs158 [eft-3p::AtTIR1(F79G)::mRuby] II. Single copy insertion into ttTi5605 on LG II. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218.
HS3545 osIs158 II; ieSi58 IV. C. elegans osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Yesbolatova A, et al. Nat Commun. 2020 Nov 11;11(1):5701.
HS3750 ieSi58 IV; osIs182 V. C. elegans ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. osIs182 [eft-3p::AtTIR1(F79G) + LoxP + myo-2p::GFP + rps-27p::neoR + LoxP] V. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. osIs182 is a single copy insertion of TIR1(F79G) into chromosome V (oxTi365) and expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218.
HS399 let-526(os37) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT and GFP+. os37 homozygotes are Lvl and Psa. Pick GFP+ heterozygotes to maintain. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57.
HS411 ceh-20(os39) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT and GFP+. Segregate GFP- sterile Unc Psa (phasmid socket absent), very rare homozygous hT2 glowing animals, and dead eggs. ceh-20(os39) animals show defects in asymmetric T cell division.
HS428 dpy-22(os26) X; osEx89. C. elegans osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-.
HS445 dpy-22(os38) X; osEx89. C. elegans osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-.
HS458 let-19(os33)/mIn1 [dpy-10(e128) mIs14] II. C. elegans Heterozygotes are WT GFP+ and segregate WT GFP+, Dpy GFP+ (mIn1 homozygotes), and os33 homozygotes (GFP-, Dpy, Muv, Steriles).
HS616 osEx108. C. elegans osEx108 [(pAY105) let-19::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. Reference: Yoda et al. Development (2005) vol. 132 (8) pp. 1885-93.
HS634 rnt-1(os58) I. C. elegans Phasmid socket absent.
HS661 nob-1(os68) III. C. elegans Healthy. Abnormal morphology of the tail (only at Nomarski level). Defects in asymmetric T cell division causes Psa (phasmid socket absent).
HS732 wrm-1(tm514) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm514 homozygotes (probable embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain.
HS845 osEx138. C. elegans osEx138 [let-526::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57.
HS886 bet-1(os46) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans Heterozygotes are WT and GFP in the pharynx, and segregate WT GFP+, Sterile Psa(Phasmid socket absent) non-GFP homozygous os46) animals and dead eggs. Maintain by picking GFP progeny. Reference: Shibata et al. Development in press (2010).
HS942 unc-76(e911) V; osEx158. C. elegans osEx158 [wrm-1::GFP + unc-76(+)]. Animals with the array are WT and GFP+. Animals which have lost the array are Unc and GFP-.
temp_name206 rmd-1(os21) III. Problem with genotype, per H Sawa

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
os1 Allele deletion
os6 Allele
os8 Allele deletion
os13 Allele substitution
os22 Allele substitution
os37 Allele
os39 Allele
os26 Allele substitution
os38 Allele substitution
os33 Allele substitution nonsense
os58 Allele substitution nonsense
os68 Allele
os46 Allele substitution nonsense