Strain Information
| Name | HS3545 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | osIs158 II; ieSi58 IV. |
| Description | osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::AID*::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Takefumi Negishi |
| Laboratory | HS |
| Reference | Yesbolatova et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nature Communications 11, 5701(2020) |
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