Strain Information
| Name | HS3750 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ieSi58 IV; osIs182 V. |
| Description | ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. osIs182 [eft-3p::AtTIR1(F79G) + LoxP + myo-2p::GFP + rps-27p::neoR + LoxP] V. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. osIs182 is a single copy insertion of TIR1(F79G) into chromosome V (oxTi365) and expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Takefumi Negishi |
| Laboratory | HS |
| Reference | Takefumi Negishi, Saho Kitagawa, Natsumi Horii, Yuka Tanaka, Nami Haruta, Asako Sugimoto, Hitoshi Sawa, Ken-ichiro Hayashi, Masahiko Harata, Masato T Kanemaki, The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans, Genetics, 2021;, iyab218, https://doi.org/10.1093/genetics/iyab218 |
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