Laboratory Information
| Name | BOX View on WormBase |
|---|---|
| Allele designation | mib |
| Head | Mike Boxem |
| Institution | Utrecht University |
| Address | Utrecht University Kruytgebouw O508 Winthontlaan 30C Utrecht 3526 KV Netherlands |
| Website | http://web.science.uu.nl/developmentalbiology/boxem/index.html |
| Gene classes | maph |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BOX163 | erm-1(mib9[erm-1[T544D]]) I. | C. elegans | Homozygous viable. Modification of endogenous erm-1 locus mimics ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX165 | erm-1(mib10[erm-1[T544A]]) I. | C. elegans | Homozygous viable. Modification of endogenous erm-1 locus mimics non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX188 | maph-1.1(mib12[GFP::maph-1.1]) I. | C. elegans | Endogenous maph-1.1 locus tagged with GFP using CRISPR/Cas9. Animals are homozygous viable and express GFP::maph-1.1 ubiquitously. Reference: Waaijers S, et al. BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x. PMID: 27506200 |
| BOX213 | erm-1(mib15[erm-1::eGFP]) I. | C. elegans | Endogenous erm-1 locus tagged with eGFP. Homozygous viable, partially functional endogenous erm-1 tag. erm-1::GFP animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. |
| BOX215 | erm-1(mib16[erm-1[T544D]::GFP]) I. | C. elegans | Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
| BOX218 | erm-1(mib19[erm-1[T544A]::GFP]) I. | C. elegans | Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 |
This laboratory hasn't submitted any alleles to the CGC.