Strain Information
| Name | SUR10 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rubSi6 II; rubSi7 IV. |
| Description | rubSi6 [eft-3p::scFv(glo)::GFP(smu-1 introns)::tbb-2 3’UTR] II. rubSi7 [eft-3p::24xGCN4::T2A::tagBFP::H2B::tbb-2 3’UTR]) IV. SunTag system allows visualization of translation throughout development in real-time. This strain expresses a SunTag reporter mRNA (24xGCN4::T2A::tagBFP::H2B::tbb-2 3’UTR ) and a SunTag antibody (scFv::GFP). In the absence of translation, GFP signal can be observed in the cytoplasm, nuclei and at epithelial apical membranes. At translation sites of the SunTag reporter, clustering of the GFP signal can be observed. Mature GCN4 proteins can be observed as dimmer GFP clusters. rubSi6 was inserted into ttTi5605 and also includes a partial duplication of the transgene (a portion of GFP with smu-1 introns and tbb-2 3’UTR) downstream of the tbb-2 3’UTR in the intact transgene. rubSi7 was inserted into cxTi10816. Reference: van der Salm E, et al. Development. 2025 May 15;152(10):dev204435. doi: 10.1242/dev.204435. PMID: 40260543. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | Suzan Ruijtenberg |
| Laboratory | BOX |
| Reference | van der Salm, E., Koelewijn, E., Schroeder, M., van der Maas, E., Jarosinska, O., Eeken, M. and Ruijtenberg, S. (2025). Measuring and manipulating localized translation of erm-1 in the C. elegans embryo. Development 152(10) https://doi.org/10.1242/dev.204435 |
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