Laboratory Information
| Name | BB View on WormBase |
|---|---|
| Allele designation | uu |
| Head | Bass, Brenda |
| Institution | University of Utah, Salt Lake City, UT |
| Address | 15 N Medical Drive East Rm 4720 Salt Lake City 84112 United States |
| Website | http://www.biochem.utah.edu/bass |
| Gene classes | adr rncs |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BB1 | dcr-1(ok247)/unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT heterozygote, Uncs (unc-32 homozygotes), and Steriles (dcr-1 homozygotes). |
| BB19 | adr-1(tm668) I. | C. elegans | Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. |
| BB2 | adr-1(gv6) I. | C. elegans | Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. |
| BB21 | adr-1(tm668) I; adr-2(ok735) III. | C. elegans | Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. |
| BB22 | adr-2(gv42) rde-4(ne299) III. | C. elegans | RNAi deficient. Maintain under normal conditions. |
| BB23 | adr-1(gv6) I; adr-2(gv42) III; rde-1(ne219) V. | C. elegans | RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Knight SW & Bass BL. Mol Cell. 2002 Oct;10(4):809-17. |
| BB239 | adr-1(uu49) I; adr-2(uu28) III. | C. elegans | Chemotaxis deficient. Transgenes are silenced in this background. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. NOTE: In the referenced publication, irregularities were noted in the adr-1(gv6);adr-2(gv42) null strain, which were ascribed to background mutationsint hat strain. This strain -- adr-1(uu49);adr-2(uu28) -- was generated Crispr/Cas9 targeted mutation and phenotypes are more consistent with another null strain, adr-1(tm668);adr-2(ok735). |
| BB24 | adr-1(gv6) I; adr-2(gv42) rde-4(ne299) III. | C. elegans | RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. |
| BB242 | adr-1(uu49) I; adr-2(uu28) III; rde-1(uu51) V. | C. elegans | RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB244 | adr-1(uu49) I; adr-2(uu28) rde-4(uu53) III. | C. elegans | RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB259 | adr-1(uu49) I; adr-2(uu28) III; ggIs1 IV. | C. elegans | ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB261 | adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III. | C. elegans | Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB270 | adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; rde-1(uu51) V. | C. elegans | RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB272 | adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) rde-4(uu53) III. | C. elegans | RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB278 | adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; ggIs1 IV. | C. elegans | ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB283 | adr-1(uu49) I; adr-2(uu28) III; ergo-1(uu68) V. | C. elegans | Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. |
| BB3 | adr-2(gv42) III. | C. elegans | Defective chemotaxis to volatile odorant. |
| BB4 | adr-1(gv6) I; adr-2(gv42) III. | C. elegans | Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. |
| BB92 | dcr-1(ok247) III; uuEx18. | C. elegans | uuEx18 [dcr-1(wild-type) + dpy-30::mCherry]. Superficially wild-type. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB93 | dcr-1(ok247) III; uuEx19. | C. elegans | uuEx19 [dcr-1(K39A) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB94 | dcr-1(ok247) III; uuEx20. | C. elegans | uuEx20 [dcr-1(D145N) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB95 | dcr-1(ok247) III; uuEx21. | C. elegans | uuEx21 [dcr-1(G492R) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
This laboratory hasn't submitted any alleles to the CGC.