| JH1521 |
C. elegans |
puf-8(ok302) II. Show Description
100% Sterile at 25C. Him and 50% Emb at 20C. Maintain at 15C.
|
|
| JK3231 |
C. elegans |
puf-8(q725) II. Show Description
Low penetrance (<10%) Mog. Do not distribute this strain; other labs should request it from the CGC.
|
|
| JK3961 |
C. elegans |
puf-8(q725)/mIn1[mIs14 dpy-10(e128)] II; lip-1(zh15) IV. Show Description
Pick wild-type GFP+ to maintain. q725 heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+ q725 heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP puf-8(q725) II; lip-1(zh15) IV double homozygotes. Homozygous puf-8(q725) II; lip-1(zh15) IV double mutants are ~100% Mog at 20C and ~100% Tumerous at 25C. Reference: Morgan CT, et al. Nat Chem Biol. 2010 Feb;6(2):102-4.
|
|
| IT540 |
C. elegans |
gap-3(kp1) I; puf-8(zh17) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are wild type and segregate wild type heterozygotes, paralyzed DpyUncs, and Uncs with germline tumors. Pick WT and check segregation of progeny to maintain. Reference: Vaid S, et al. Development. 2013 Apr;140(8):1645-54.
|
|
| JK6063 |
C. elegans |
puf-8(q1048[3xV5::puf-8]) II. Show Description
3xV5 tag inserted into endogenous puf-8 locus via CRISPR/Cas9 engineering. Reference: Ferdous AS, et al. Development. 2023 May 1;150(9):dev201705. PMID: 37070766.
|
|
| SHG1614 |
C. elegans |
puf-8(ust245[puf-8::GFP::3xFlag]) II. Show Description
GFP::3xFlag inserted into endogenous puf-8 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
|
|
