| OK461 |
C. elegans |
bcl-11(cu10)/unc-46(e177) dpy-11(e224) V. Show Description
Pick wild-type to maintain. Heterozygotes are wild-type and should segregate wild-type heterozygotes, bcl-11 homozygotes (L1 larval arrest with starved appearance), and Dpy Unc homozygotes (Medium Dpy, Shrinker, poor backing). Maintain by picking wild-type and scoring for proper segregation of progeny. bcl-11 homozygotes have weak pharyngeal muscle contractions and pharyngeal lumen fails to open. cu10 is a 555 bp deletion (V:6360921..6361460). Predicted bcl-11 null allele. Reference: Vilimas, Tomas. (2004). Genes regulating ceh-22 and pharyngeal development of Caenorhabditis elegans.. University of Illinois at Chicago. Thesis. https://hdl.handle.net/10027/12060
|
|
| RB699 |
C. elegans |
rgs-9(ok461) X. Show Description
ZC53.7, ZC53.6. Homozygous. Outer Left Sequence: GGCACAGTCTGCAAGATGAA. Outer Right Sequence: ATCCACAACTCCATGGCTTC. Inner Left Sequence: GTCATTCACACACACAGCCC. Inner Right Sequence: CAAATGCCGAGTAAGGGAAA. Inner primer WT PCR product: 2766. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
|
|
