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|Species|| C. elegans |
|Genotype||daf-16(mu86) I; daf-2(e1370) III; hpEx3370.|
|Description||hpEx3370 [dpy-30p::daf-16a::unc-54 3' UTR + myo-2p::mCherry]. Pick RFP+ and keep at 15C to maintain. Can be kept at 20C, but some animals will form dauers. Transgenic strain expressing DAF-16A isoform from pan-tissue dpy-30 promoter. Transgenic animals have RFP in pharynx and will form dauers at 25C. Non-transgenic animals are dauer-defective (Daf-d). Reference: Hung WL. et al., Development (2014) 141 (8): 1767–1779.|
|Made by||Wesley Hung|
|Reference ||Hung et al.,
Development (2014) 141 (8): 1767–1779.