Strain Information
| Name | ZM8607 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | hpIs481. |
| Description | hpIs481 [ceh-12p::tomm20::miniSOG::SL2::BFP + unc-129(DB)p::tomm20::miniSOG::SL2::BFP + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2), induces mitochondrial-miniSOG ablation of B-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat disipator to keep the air cool. unc-129(DB)p is a fragment of the unc-129 promoter driving expression in only DB motor neurons (described in Colavita et al., Science 1998 31;281(5377):706-9). The ceh-12 promoter drives expression in VB motor neurons. Generated in N2 background. Reference: Gao S, et al. eLife 2018 Jan 23;7:e29915. doi: 10.7554/eLife.29915. |
| Mutagen | UV Rays |
| Outcrossed | x4 |
| Made by | Mei Zhen |
| Laboratory | ZM |
| Reference | Gao, S. et al. Excitatory motor neurons are local oscillators for backward locomotion. Elife7, e29915 (2018). |
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