Strain Information

Name ZM11020   View On Wormbase
Species C. elegans
GenotypeljIs131; hpEx4343.
DescriptionljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. hpEx4343 [acr-5p::TeTx::wCherry + unc-4p::TeTx::wCherry + HygromycinR]. Pick animals with wCherry expression in ventral cord neurons to maintain hpEx4343. Animals carrying the array show additional red fluorescence in the head compared to those that have lost the array. Animals carrying hpEx4343 rest as coilers strongly biased towards ventral bend as L1 larvae and are severely Unc as adults. Coiling is somewhat suppressed in the ljIs131 background, but animals still exhibit an obvious bias towards ventral bend during movement. Hygromycin can be used to select for hpEx4343 transgenic animals. Reference: Lu Y, et al. Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 10.1016/j.cub.2022.09.002. PMID: 36182701.
Made byYan Li
Laboratory ZM
Reference Lu Y, Ahamed T, Mulcahy B, Witvliet D, Guan SA, Hung W, Meng J, Wen Q, Samuel ADT, Zhen M.*. Extrasynaptic signaling enables an asymmetric juvenile motor circuit to produce a symmetric gait. Current Biology. In Press
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