Strain Information

Name XE1269   View On Wormbase
Species C. elegans
GenotypejuIs76 II; rib-2(gk318) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
DescriptionjuIs76 [unc-25p::GFP + lin-15(+)] II. Homozygous viable or sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk318 homozygotes (often sterile, lays eggs that don't hatch; some eggs hatch and develop to fertile adulthood). Homozygous hT2 [bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT with pharyngeal GFP and check for correct segregation of progeny to maintain. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
MutagenNo mutagen
Outcrossedx0
Made byTyson Edwards
Laboratory XE
Reference Edwards TJ, Hammarlund M. Syndecan promotes axon regeneration by stabilizing growth cone migration. Cell reports. 2014; 8(1):272-83. NIHMSID: NIHMS606077 PubMed [journal] PMID: 25001284, PMCID: PMC4127196
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