Strain Information

Name WHY219   View On Wormbase
Species C elegans
Genotypeeggd-1(how5) III; pgl-1(gg547[pgl-1::3x flag::tagRFP]) IV
Descriptionhow5 is a full deletion of eggd-1(C38D4.4) generated using CRISPR-Cas9. In this background PGL-1::tagRFP becomes detached from the nuclear envelope in the germ line and forms large cytoplasmic foci. PGL-1::tagRFP also does not partition efficiently to the P lineage in embryos. Strain exhibits reduced brood size and becomes sterile over successive generations at 20 C. Fertility defects are greatly exacerbated by increase in temperature. eggd-1 is also independently reported as mip-1.
Mutagen Crispr/Cas9
Made byWen Tang
Laboratory WHY
Reference Ian F Price, Hannah L Hertz, Benjamin Pastore, Jillian Wagner, Wen Tang (2021) Proximity labeling identifies LOTUS domain proteins that promote the formation of perinuclear germ granules in C. elegans eLife 10:e72276
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