Laboratory Information
| Name | WHY View on WormBase |
|---|---|
| Allele designation | how |
| Head | Wen Tang |
| Institution | The Ohio State University |
| Address | 1060 Carmack Road Rightmire Hall Room 218 Columbus 43210 United States |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| WHY178 | pgl-1(gg547[pgl-1::3x flag::tagRFP]) IV; eggd-2(how6) V | C. elegans | how6 is a random 17 nt insertion after the eggd-2 (F58G11.3) start codon. The allele is presumably null, but at least a strong hypomorph. PGL-1::tagRFP localization in the germ line is apparently wildtype but is defective in embryos. Particularly after the first 1-2 rounds of cell division, PGL-1::tagRFP does not robustly localize to the P lineage. Strain becomes sterile over successive generations and is less fertile than wildtype. eggd-2 is also independently reported as mip-2. |
| WHY200 | mip-1(how7[mip-1::GFP::TEV::3xFLAG::AID]) III; mip-2(how16[mCherry::mip-2]) V. | C. elegans | GFP::TEV::3xFLAG::AID tag inserted into the C-terminus of endogenous mip-1 locus using CRISPR. mCherry tag inserted into the N-terminus endogenous mip-2 locus using CRISPR. MIP-1::GFP and mCherry::MIP-2 localize to P granules in the germ line and in embryos. mip-1 also known as eggd-1/C38D4.4. mip-2 also known as eggd-2/F58G11.3. Reference: Price IF, et al. Elife. 2021 Nov 3;10:e72276. doi: 10.7554/eLife.72276. PMID: 34730513. |
| WHY219 | eggd-1(how5) III; pgl-1(gg547[pgl-1::3x flag::tagRFP]) IV | C elegans | how5 is a full deletion of eggd-1(C38D4.4) generated using CRISPR-Cas9. In this background PGL-1::tagRFP becomes detached from the nuclear envelope in the germ line and forms large cytoplasmic foci. PGL-1::tagRFP also does not partition efficiently to the P lineage in embryos. Strain exhibits reduced brood size and becomes sterile over successive generations at 20 C. Fertility defects are greatly exacerbated by increase in temperature. eggd-1 is also independently reported as mip-1. |
This laboratory hasn't submitted any alleles to the CGC.