Laboratory Information

NameWHY View on WormBase
Allele designationhow
HeadWen Tang
InstitutionThe Ohio State University
Address 1060 Carmack Road
Rightmire Hall Room 218
Columbus 43210
United States
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
WHY178 pgl-1(gg547[pgl-1::3x flag::tagRFP]) IV; eggd-2(how6) V C. elegans how6 is a random 17 nt insertion after the eggd-2 (F58G11.3) start codon. The allele is presumably null, but at least a strong hypomorph. PGL-1::tagRFP localization in the germ line is apparently wildtype but is defective in embryos. Particularly after the first 1-2 rounds of cell division, PGL-1::tagRFP does not robustly localize to the P lineage. Strain becomes sterile over successive generations and is less fertile than wildtype. eggd-2 is also independently reported as mip-2.
WHY200 mip-1(how7[mip-1::GFP::TEV::3xFLAG::AID]) III; mip-2(how16[mCherry::mip-2]) V. C. elegans GFP::TEV::3xFLAG::AID tag inserted into the C-terminus of endogenous mip-1 locus using CRISPR. mCherry tag inserted into the N-terminus endogenous mip-2 locus using CRISPR. MIP-1::GFP and mCherry::MIP-2 localize to P granules in the germ line and in embryos. mip-1 also known as eggd-1/C38D4.4. mip-2 also known as eggd-2/F58G11.3. Reference: Price IF, et al. Elife. 2021 Nov 3;10:e72276. doi: 10.7554/eLife.72276. PMID: 34730513.
WHY219 eggd-1(how5) III; pgl-1(gg547[pgl-1::3x flag::tagRFP]) IV C elegans how5 is a full deletion of eggd-1(C38D4.4) generated using CRISPR-Cas9. In this background PGL-1::tagRFP becomes detached from the nuclear envelope in the germ line and forms large cytoplasmic foci. PGL-1::tagRFP also does not partition efficiently to the P lineage in embryos. Strain exhibits reduced brood size and becomes sterile over successive generations at 20 C. Fertility defects are greatly exacerbated by increase in temperature. eggd-1 is also independently reported as mip-1.
This laboratory hasn't submitted any alleles to the CGC.