Strain Information
| Name | VBS664 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | vbaIs56 I; nrde-2(gg95) vbaIs55 II; eri-1(mg366) IV. |
| Description | vbaIs56 [eef-1A.1p::VenusN::nrde-3] I. vbaIs55 [eef-1A.1p::VenusC::nrde-3] II. Maintain at 20C or cooler; germline mortal (Mrt) at 25C. N-terminal and C-terminal fragments of the fluorescent protein Venus are fused to NRDE-3 to facilitate trimolecular fluorescence complementation. In the cytoplasm or nucleus, local concentration of NRDE-3 molecules does not allow fluorescence complementation, thus reducing background fluorescence; once bound on the target transcript, VenusN::NRDE-3 and VenusC::NRDE-3 are in sufficient proximity to allow for fluorescence complementation, labeling transcription sites of dsRNA gene targets. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044. |
| Mutagen | No mutagen |
| Outcrossed | x0 |
| Made by | A. Barriere |
| Laboratory | VBS |
| Reference | Imaging of native transcription and transcriptional dynamics in vivo using a tagged Argonaute protein. Amel Toudji-Zouaz, Vincent Bertrand, Antoine Barrière Nucleic Acids Research, gkab469, https://doi.org/10.1093/nar/gkab469 |
Sign in
or
register an account if you want to order this strain.