Laboratory Information
| Name | VBS View on WormBase |
|---|---|
| Allele designation | vba |
| Head | Bertrand, Vincent |
| Institution | Institute of Developmental Biology of Marseilles (IBDM), Marseille, France |
| Address | IBDM UMR7288 CNRS Parc Scientifique de Luminy Case 907 Marseille cedex 9 13288 France |
| Website | http://www.ibdml.univ-mrs.fr |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| OH10162 | otEx4503. | C. elegans | otEx4503 [hlh-16::GFP + rol-6(su1006)]. Translation HLH-16::GFP reporter. Pick Rollers to maintain. Reference: Bertrand et al. (2011) Current Biology 21, 1225-1231. |
| OH9345 | otEx4140. | C. elegans | otEx4140 [hlh-3(fosmid)::YFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Reference: Murgan et al. (2015) Developmental Cell 33, 737-745. |
| VBS662 | nrde-2(gg95) vbaIs52 II; eri-1(mg366) IV. | C. elegans | vbaIs52 [eef-1A.1p::YFP::nrde-3] II. Maintain at 20C or cooler; germline mortal (Mrt) at 25C. YFP::NRDE-3 localizes to the cytoplasm, except in the germline, early embryo, and intestine. Upon introduction of dsRNA, YFP::NRDE-3 labels transcription sites of dsRNA gene targets. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044. |
| VBS663 | nrde-2(gg95) vbaIs53 II; eri-1(mg366) IV. | C. elegans | vbaIs53 [rps-27p::mNeonGreen::Flag::nrde-3] II. Maintain at 20C or cooler; germline mortal (Mrt) at 25C. mNG::NRDE-3 localizes to the cytoplasm. Upon introduction of dsRNA, mNG::NRDE-3 labels transcription sites of dsRNA gene targets. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044. |
| VBS664 | vbaIs56 I; nrde-2(gg95) vbaIs55 II; eri-1(mg366) IV. | C. elegans | vbaIs56 [eef-1A.1p::VenusN::nrde-3] I. vbaIs55 [eef-1A.1p::VenusC::nrde-3] II. Maintain at 20C or cooler; germline mortal (Mrt) at 25C. N-terminal and C-terminal fragments of the fluorescent protein Venus are fused to NRDE-3 to facilitate trimolecular fluorescence complementation. In the cytoplasm or nucleus, local concentration of NRDE-3 molecules does not allow fluorescence complementation, thus reducing background fluorescence; once bound on the target transcript, VenusN::NRDE-3 and VenusC::NRDE-3 are in sufficient proximity to allow for fluorescence complementation, labeling transcription sites of dsRNA gene targets. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044. |
| VBS668 | nrde-2(gg95) vbaIs54 II; eri-1(mg366) IV. | C. elegans | vbaIs54 [eef-1A.1p::YFP::nrde-3::SL2::sid-1] II. YFP::NRDE-3 localizes to the cytoplasm in most somatic tissues and upon exposure to dsRNA targetting a gene, moves to the nucleus in cells expressing the transgene. Superficially wild-type. Reference: Toudji-Zouaz A, et al. Nucleic Acids Research. 2021 Jun 9;gkab469. doi: 10.1093/nar/gkab469. PMID: 34107044. |
This laboratory hasn't submitted any alleles to the CGC.