Strain Information

Name UDN100163   View On Wormbase
Species C. elegans
Genotypepph-5(udn93) V.
Descriptionpph-5 [A48A] #1 control edit for strain UDN100160 from Fielder, et al. (2022). Wild-type sequence except for addition of silent AvaII restriction site and silent PAM ablation. Homozygous viable and wild-type appearance. Genotype using primers Forward: accggaaattgtcccaaaat, Reverse: tttccgactttctggaggtg. Digest with AvaII restriction enzyme for resulting sizes of 401bp +403bp. Wild-type product will not be digested.
MutagenCrispr/Cas9
Outcrossedx2
Made byUDN Screening Center
Laboratory UDN
Reference Fielder et al, 2022. Mol Genet Metab, In Press
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