Strain Information

Name UDN100028   View On Wormbase
Species C. elegans
Genotyperab-5(udn14)/tmC18 [dpy-5(tmIs1200)] I.
DescriptionMust be maintained at >20 degrees; grows better at 25C. Homozygous lethal rab-5 [D135H] mutation balanced by tmC18. Balancer marked with myo-2p::Venus. Heterozygotes are WT with pharyngeal Venus fluorescence, and segregate Venus+ heterozygotes, non-Venus rab-5[D135H] homozygotes (L1 lethal), and Dpy Venus+ tmC18 homozygotes. Pick fertile wild-type Venus+ to maintain. Silent BstAPI site added in D135H for genotyping ease. Heterozygous rab-5[D135H] animals are small and have decreased locomotion. Reference: Huang et al. 2022. PMID: 35121658
MutagenCrispr/Cas9
Outcrossedx3
Made byUDN Screening Center
Laboratory UDN
Reference Huang et al. 2022. PMID: 35121658
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