Strain Information
| Name | SSM264 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rad-51(iow53[GFP::rad-51]) IV/nT1[qIs51] (IV;V). |
| Description | Heterozygotes are wild-type with pharyngeal-expressed GFP, and segregate wild-type GFP+(pharynx) heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP(pharynx) rad-51(iow53[GFP::rad-51]) homozygotes. Balancer is prone to breaking down. If a population contains a mix of bright and dim GFP animals, pick dim GFP and check for correct segregation of progeny to maintain. iow53 inserted a GFP tag at the N-terminus of the endogenous rad-51 locus, but the tagged protein is not fully functional. non-GFP(pharynx) rad-51(iow53[GFP::rad-51]) homozygotes form GFP foci in the germline that are mostly spo-11 dependent, and GFP::rad-51 homozygotes have defects in unloading RAD-51. Created by CRISPR using pDD282, therefore may also contain 3XFLAG. Reference: Koury E, et al. Nucleic Acids Res. 2018 Jan 25;46(2):748-764. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Matthew Wheat and Emily Koury |
| Laboratory | SSM |
| Reference | Koury E, Harrell K, Smolikove S. Nucleic Acids Res. 2018 Jan 25;46(2):748-764. |
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