Strain Information

Name QC152   View On Wormbase
Species C. elegans
Genotypemdt-15(et14) III.
DescriptionThe mdt-15(et14) gain-of-function mutation has no obvious phenotype on its own but can act as a paqr-2 suppressor. The C5832666T [WS200] mutation can be detected by PCR using the following primers: mdt-15(et14) Mut Fwd: 5’-GTGCCTCCAGATCCACAGCT-3’; mdt-15(et14) WT Fwd: 5’-GTGCCTCCAGATCCACAGCC-3’; mdt-15 Rev: 5’-CACCCATTGGAGCACCACT-3’. Annealing 65°C, expected product ~400 bp. Reference: Svensk E, et al. PLoS Genetics 9:e1003801. PMID: 24068966
MutagenEMS
Outcrossedx10
Made byRakesh Bodhicharla
Laboratory QC
Reference Svensk, E., Ståhlman, M., Andersson, C.-H., Johansson, M., Borén, J. and Pilon, M. (2013). PAQR-2 regulates fatty acid desaturation during cold adaptation in C. elegans. PLoS Genetics 9:e1003801.
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