Strain Information

Name PS9496   View On Wormbase
Species C. elegans
Genotypehim-5(e1490) V; seb-3(sy1794) X.
DescriptionSuperficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of seb-3 in him-5(e1490) background. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GAATTGAAAAAACTGGAAAACAGTTCGTATAATCCG right flanking sequence: GGgtgggtcaagttccagtgttcagtttttttaaag inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: ACAGTTCGTATAATCCGGGG Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
MutagenCrispr/Cas9
Outcrossedx0
Made byHeenam Park
Laboratory PS
Reference n/a
Sign in or register an account if you want to order this strain.