Strain Information

Name PS8661   View On Wormbase
Species C. elegans
GenotypesyIs691; syIs300.
DescriptionsyIs691 [pdf-1p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR + grl-2p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. Split cGAL driver for MCM neuron. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation.
Mutagen
Made byChun-Hao Chen/Stephanie Nava
Laboratory PS
Reference Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW. cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. Nat Methods. 2017 Feb;14(2):145-148. doi: 10.1038/nmeth.4109. Epub 2016 Dec 19. PubMed PMID:27992408
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