Strain Information
| Name | PS8661 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | syIs691; syIs300. |
| Description | syIs691 [pdf-1p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR + grl-2p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]. Split cGAL driver for MCM neuron. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation. |
| Mutagen | |
| Made by | Chun-Hao Chen/Stephanie Nava |
| Laboratory | PS |
| Reference | Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW. cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. Nat Methods. 2017 Feb;14(2):145-148. doi: 10.1038/nmeth.4109. Epub 2016 Dec 19. PubMed PMID:27992408 |
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