Strain Information

Name PS8450   View On Wormbase
Species C. elegans
Genotypenpr-27(sy1315) IV.
DescriptionSuperficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of npr-27. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: gggtgtgccttcttgATGGAGGATTTTTCCTCGA right flanking sequence: ATTTCACGACAACTTCAATTCAGAATGATAGTTATG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TGAAGTTGTCGTGAAATTCG Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616
MutagenCrispr/Cas9
Outcrossedx0
Made byHeenam Park & Mandy Tan
Laboratory PS
Reference n/a
Sign in or register an account if you want to order this strain.