Strain Information
Name | PS8450 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | npr-27(sy1315) IV. |
Description | Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of npr-27. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: gggtgtgccttcttgATGGAGGATTTTTCCTCGA right flanking sequence: ATTTCACGACAACTTCAATTCAGAATGATAGTTATG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TGAAGTTGTCGTGAAATTCG Method Reference: G3 (Bethesda). 2018 Nov 6;8(11):3607-3616 |
Mutagen | Crispr/Cas9 |
Outcrossed | x0 |
Made by | Heenam Park & Mandy Tan |
Laboratory | PS |
Reference | n/a |
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