Strain Information
| Name | PQ535 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | alg-1(ap428 [alg-1::Y45F10D.4 3'UTR]) X. |
| Description | alg-1(ap428 [alg-1::Y45F10D.4 3’UTR]) X. alg-1 control strain. ap428 is a CRISPR-engineered allele in which the endogenous alg-1 3'UTR was replaced by the Y45F10D.4 3'UTR. The Y45F10D.4 3'UTR was chosen because it appears to be stably expressed, is commonly used as a control gene in quantitative RT-PCR experiments, and its short 3’UTR lacks ALG-1 binding sites. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
| Mutagen | CRISPR/Cas-9 |
| Outcrossed | x4 |
| Made by | Antti Aalto |
| Laboratory | PQ |
| Reference | Aalto AP, Nicastro IA, et al (2018). PLoS Genet. 14(6) e1007379 |
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