Laboratory Information
| Name | PQ View on WormBase |
|---|---|
| Allele designation | ap |
| Head | Pasquinelli, Amy |
| Institution | University of California, San Diego, CA |
| Address | 9500 Gilman Drive 4105 Bonner Hall MC0349 La Jolla 92093 United States |
| Website | http://www.biology.ucsd.edu/labs/pasquinelli/index.html |
| Gene classes | rbm cest |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| PQ320 | apIs320 II; unc-119(ed3) III. | C. elegans | apIs320 [let-7::unc-119(+)] II. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ402 | apIs402 II; unc-119(ed3) III. | C. elegans | apIs402 [let-7(delta alg-1-binding site)::unc-119(+)] II. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ404 | apIs404 II; unc-119(ed3) III. | C. elegans | apIs404 [let-7(delta alg-1-binding site)::unc-119(+)] II. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ425 | apIs320 II; unc-119(ed3) III; unc-3(e151) let-7(mn112) X. | C. elegans | apIs320 [let-7::unc-119(+)] II. PQ425 was created by crossing PQ320 into unc-3(e151) let-7(mn112) animals, which do not express precursor or mature let-7. unc-119(ed3) might not be homozygous in this strain. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ426 | apIs404 II; unc-119(ed3) III; unc-3(e151) let-7(mn112) X. | C. elegans | apIs404 [let-7(delta alg-1-binding site)::unc-119(+)] II. PQ426 was created by crossing PQ404 into unc-3(e151) let-7(mn112) animals, which do not express precursor or mature let-7. unc-119(ed3) might not be homozygous in this strain. Reference: Zisoulis DG, et al. Nature. 2012;486(7404):541-544. |
| PQ530 | alg-1(ap423[3xflag::gfp::alg-1]) X. | C. elegans | alg-1(ap423 [3xflag::gfp::alg-1]) X. ALG-1 tagged at N-terminal with 3xFLAG:GFP at endogenous locus, verified by western blot and fluorescence microscopy. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
| PQ535 | alg-1(ap428 [alg-1::Y45F10D.4 3'UTR]) X. | C. elegans | alg-1(ap428 [alg-1::Y45F10D.4 3’UTR]) X. alg-1 control strain. ap428 is a CRISPR-engineered allele in which the endogenous alg-1 3'UTR was replaced by the Y45F10D.4 3'UTR. The Y45F10D.4 3'UTR was chosen because it appears to be stably expressed, is commonly used as a control gene in quantitative RT-PCR experiments, and its short 3’UTR lacks ALG-1 binding sites. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
| PQ567 | alg-2(ap426) II. | C. elegans | ap426 is a CRISPR-engineered 8 bp deletion in the ALG-2 isoform A exon 2 causing a frameshift that produces a heavily truncated protein. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
| PQ582 | alg-2(ap431[3xflag::mKate2::alg-2]) II. | C. elegans | alg-2(ap431[3xflag::mKate2::alg-2]) II. ALG-2 tagged at N-terminal with 3xFLAG:mKate2 at endogenous locus, verified by western blot and fluorescence microscopy. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
| PQ583 | alg-2(ap431[3xflag::mKate2::alg-2]) II; alg-1(ap423[3xflag::gfp::alg-1]) X. | C. elegans | alg-2(ap431[3xflag::mKate2::alg-2]) II. alg-1(ap423[3xflag::gfp::alg-1]) X. Derived from crossing PQ530 and PQ582 strains, verified by fluorescence microscopy. Reference: Aalto AP, et al. PLoS Genet. 2018 Jun 21;14(6):e1007379. |
This laboratory hasn't submitted any alleles to the CGC.