Strain Information

Name PE874   View On Wormbase
Species C. elegans
GenotypefeIs5 X; dvIs15.
DescriptionfeIs5 [sur-5p::luciferase::GFP + rol-6(su1006)] X. dvIs15 [(pPD30.38) unc-54(vector) + (pCL26) mtl-2::GFP]. Rollers. Strain is bioluminescent when provided with exogenous D-luciferin (potassium salt) due to sur-5 promoter driving expression of firefly (Photinus pyralis) luciferase (lacking the peroxisome tagging signal) fused in-frame to GFP(S65C). Pick animals with high levels of fluorescence to retain expression of luciferase transgene. This strain is for academic use only. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. References: Lagido C, et al. BMC Physiol. 2008 Apr 2;8:7. McLaggan D, et al. PLoS One. 2012;7(10):e46503. Lagido C, et al. Toxicol Sci. 2009 May;109(1):88-95.
MutagenNo mutagen
Made byCristina Lagido
Laboratory PE
Reference Derivative of PE255 described in Lagido C et al. BMC Physiol. 2008 Apr 2; Lagido C et al. 2009.Toxicological Sciences 109(1), 88–95; McLaggan D et al. 2012. PLoS ONE 7 (10); Lagido C et al. 2015 JoVE (104), e53083, doi:10.3791/53083; Olmedo et al., 2015 Genetics 20 (2):443-448; Luz AL et al. 2016. Current Protocols in Toxicology 69:25.8.1-25.8.22
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