Strain Information
| Name | PD2557 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rps-10(cc2557)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. |
| Description | Balancer recombination happens frequently at 23-25C, strain must be maintained at 16-20C. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are non-Dpy with relatively dim pharyngeal GFP (Venus) expression, and segregate heterozygous non-Dpy Venus+, non-Venus cc2557 homozygotes (L1 arrest), and Dpy with brighter Venus+ (tmC20 homozygotes). Pick wild-type Venus(+) and check for proper segregation of progeny to maintain. cc2557 is an engineered mutation creating an early stop (T8*). Presumptive rps-10 null. Heterozygous rps-10(cc2557)/tmC20 animals are delayed in development. Reference: Cenik ES, et al. Dev Cell. 2019 Mar 25;48(6):811-826.e6. doi: 10.1016/j.devcel.2019.01.019. PMID: 30799226. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Elif Sarinay Cenik & Agustian Surya |
| Laboratory | ESC |
| Reference | Cenik ES, Meng X, Tang NH ,Hall RN, Arribere JA, Cenik C, Jin Y, Fire AZ (2019). Maternal ribosomes are sufficient for tissue diversification during embryonic development in C. elegans. Developmental Cell, Mar25;48(6), 811-826.e6. , highlighted in the same issue with a preview by Haag & Dinman, 2019, Featured in Faculty of 1000. |
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