Strain Information
| Name | OH17932 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | linc-26(ot1227[linc-26p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) IV. |
| Description | The Null Transcriptional Reporter (NuTR) cassette was used to remove the linc-23 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. GFP expression is seen in the male somatic gonad and extremely dim expression in hermaphrodite spermatheca. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Sandeep Wontakal |
| Laboratory | OH |
| Reference | n/a |
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