Strain Information
| Name | NWG316 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | pkc-3(crk77[I331A,T394A]) II; par-2(it328[gfp::par-2]) III. |
| Description | GFP tag inserted into endgonenous par-2 locus in an analogue-sensitive background. pkc-3(crk77[I331A,T394A]) is a CRISPR-engineered analog-sensitive allele containing both I331A (gatekeeper site) and T394A (suppressor site) mutations, allowing rapid and reversible chemical inhibition of PKC-3 activity. Reference: Ng K, et al. (2022). An analog sensitive allele permits rapid and reversible chemical inhibition of PKC-3 activity in C. elegans. Reference: Ng K, et al. microPublication Biology. 10.17912/micropub.biology.000610 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | KangBo Ng |
| Laboratory | NWG |
| Reference | Ng, K; Bland, T; Hirani, N; Goehring, NW (2022). An analog sensitive allele permits rapid and reversible chemical inhibition of PKC-3 activity in C. elegans. microPublication Biology. 10.17912/micropub.biology.000610 |
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