Strain Information
| Name | NJF01 View On Wormbase |
|---|---|
| Species | Escherichia coli |
| Genotype | NJF01 (F-, lambda- lysA0::Tn10 IN(rrnD-rrnE)1, DE3, (delta)rnc-38). |
| Description | Bacteria. NJF01 originates from E. coli ET505 (F-, lambda- lysA0::Tn10 IN(rrnD-rrnE)1), modified with DE3 and (delta)rnc-38. The E. coli is lysine auxotroph and resistant to kanamycin and tetracycline. IPTG induces expression of the T7 polymerase. Grow at 37 °C on LA plates or in LB liquid medium. Reference: Fredens J, et al. Nat Methods. 2011 Aug 28;8(10):845-7. |
| Mutagen | |
| Outcrossed | x0 |
| Made by | Julius Fredens |
| Laboratory | FE |
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