Strain Information
| Name | MV1 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | polg-1(srh1)/mnC1[dpy-10(e128) unc-52(e444) nIs190 let-?] II |
| Description | [This strain is not available to order. The strain accumulates mitochondrial defects even as a het, and is not suitable for long-term propagation.] The homozygous polg-1(srh1) allele is sterile and the worms are non fluorescent. The homozygous balancer strain is lethal. The heterozygote strain has a fluorescent pharyngeal bulb. Pick heterozygotes to maintain the strain. Over multiple generations the strain accumulates mitochondrial DNA mutations. It is best to outcross the polg-1(srh1) allele using males into a hermaphrodite that has wildtype mitochondrial DNA. The behavioral phenotypes and mitochondrial dysfunction starts around 15 generations of carrying the mutant polymerase and plateaus at around 30 generations. |
| Mutagen | CRISPR targeted mutation |
| Outcrossed | x4 |
| Made by | Marc Vermulst |
| Laboratory | MAV |
| Reference | Haroon S, Li A, Weinert JL, Fritsch C, Ericson NG, Alexander-Floyd J, Braeckman BP, Haynes CM, Bielas JH, Gidalevitz T, Vermulst M. (2018) "Multiple Molecular Mechanisms Rescue mtDNA Disease in C. elegans." Cell Rep. 22(12):3115-3125 |
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