Strain Information

Name MQD2375   View On Wormbase
Species C. elegans
Genotypedaf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III; ieSi38 IV.
DescriptionieSi38 [sun-1p::TIR1::mRuby::sun-1 3' UTR + Cbr-unc-119(+)] IV. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. A single copy transgene was inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in the germ line.
Made byYan-Ping Zhang, Wen-Hong Zhang
Laboratory MQD
Reference Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567.
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