Strain Information
| Name | MQD2375 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III; ieSi38 IV. |
| Description | ieSi38 [sun-1p::TIR1::mRuby::sun-1 3' UTR + Cbr-unc-119(+)] IV. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. A single copy transgene was inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in the germ line. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Yan-Ping Zhang, Wen-Hong Zhang |
| Laboratory | MQD |
| Reference | Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https://doi.org/10.1101/2021.07.31.454567. |
Sign in
or
register an account if you want to order this strain.