Strain Information

Name MLC903   View On Wormbase
Species C. elegans
GenotypenDf67 mir-52(n4100) IV/nT1 [qIs51] (IV;V); nDf58 X, lucIs24.
DescriptionlucIs24 [mir-52p::mirtron-51 + elt-2::dsRed + myo-2::mCherry]. Pick GFP+ animals to maintain balanced line. Balanced mir-51 family mutant expressing a mirtron-version of mir-51. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP, arrested nT1[qIs51] aneuploids, and non-GFP mir-51 family homozygous mutants. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Non-GFP mir-51 family homozygous mutants rescued by mirtron-51 transgene are viable, but slow-growing and sick. Strain is derived from injection into parental strain MT17143. lucIs24 is a spontaneous integrant originating from a complex extra-chromosomal array, the genomic location of the transgene is unknown. Reference: Dexheimer, PJ, et al. Curr Biol. 2020. in press.
Made byPhilipp Dexheimer
Laboratory MLC
Reference Dexheimer, PJ, et al. Curr Biol. 2020 in press
Sign in or register an account if you want to order this strain.