Strain Information

Name MIR276   View On Wormbase
Species C. elegans
GenotyperisIs33; gpIs1.
DescriptionrisIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. gpIs1 [hsp-16.2p::GFP]. Inducible GFP fluorescence after >1 hour heat shock at 35C. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511. Derived by crossing parental strains MIR249 and TJ375.
MutagenNo mutagen
Made byAnita Goyala
Laboratory MIR
Reference Fischer F, Benner C, Goyala A, Vitiello D, Grigolon G, Wu JY, Zarse K, Collin YE, Ristow M. Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation. J Biol Chem, doi: 10.1016/j.jbc.2022.102085 (2022) PMID: 35636511 DOI: 10.1016/j.jbc.2022.102085
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