Laboratory Information

NameMIR View on WormBase
Allele designationris
HeadMichael Ristow
InstitutionETH Zurich, Schwerzenbach, Switzerland
Address Schorenstrasse 16
Schwerzenbach 8603
Switzerland
Website http://www.energymetab.ethz.ch/
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
MIR12 risIs2. C. elegans risIs2 [anmt-1p::anmt-1::HA + unc-119(+)]. Superficially wild-type. Reference: Schmeisser K, et al. Nat Chem Biol. 2013 Sep 29. doi: 10.1038/nchembio.1352.
MIR13 sir-2.1(ok434) IV; aak-2(ok524) X. C. elegans Slow growing. Maintain under normal conditions. Derived from parental strains RB754 [aak-2(ok524)] and VC199 [sir-2.1(ok434)]. Reference: Schmeisser S, et al. Molecular Metabolism February 15, 2013. DOI: 10.1016/j.molmet.2013.02.002.
MIR22 geIs3 I; anmt-1(gk457) III. C. elegans geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. Derived from sir-2.1-overexpressing strain GA468 crossed with 5x outcrossed anmt-1(gk457) [strain MIR16]. Reference: Schmeisser K, et al. Nat Chem Biol. 2013 Sep 29. doi: 10.1038/nchembio.1352.
MIR23 risIs3. C. elegans risIs3 [K02A4.1p::K02A4.1::GFP + unc-119(+)]. Superficially wild-type. GFP mainly in head region and body wall muscle. Reference: Mansfeld J, et al. Nat Commun, 2015 Dec 1;6:10043.
MIR24 risIs4. C. elegans risIs4 [sur-5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. Superficially wild-type. Strain harbours 171 kDa dCas9-VP64 protein.
MIR249 risIs33. C. elegans risIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511.
MIR250 hif-1(ia4) V; risIs33. C. elegans risIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511. Derived by crossing parental strains MIR249 and ZG31.
MIR251 hsf-1(sy441) I; risIs33. C. elegans risIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511. Derived by crossing parental strains MIR249 and PS3551.
MIR260 risIs31. C. elegans risIs31 [hsp-16.2p::grh-1b::GFP + unc-119(+)]. Long lived without heat shock. Heat shock induces over-expression of grh-1 causing short-lived phenotype and nuclear GFP expression. Described as "hsp-16.2p::grh-1::gfp OEx line 1" in referenced paper. Reference: Grigolon G, et al. Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4. PMID: 35013237.
MIR261 risIs31. C. elegans risIs31 [hsp-16.2p::grh-1b::GFP + unc-119(+)]. Long lived without heat shock. Heat shock induces over-expression of grh-1 causing short-lived phenotype and nuclear GFP expression. Reference: Grigolon G, et al. Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4. PMID: 35013237.
MIR262 risls32. C. elegans risls32 [grh-1p::grh-1b::GFP + unc-119(+)]. Over-expression of grh-1 from its endogenous promoter. Short lived, no GFP signal visible. Reference: Grigolon G, et al. Nat Commun. 2022 Jan 10;13(1):107. doi: 10.1038/s41467-021-27732-4. PMID: 35013237.
MIR276 risIs33; gpIs1. C. elegans risIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. gpIs1 [hsp-16.2p::GFP]. Inducible GFP fluorescence after >1 hour heat shock at 35C. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511. Derived by crossing parental strains MIR249 and TJ375.
MIR8 risIs1. C. elegans risIs1 [anmt-1p::anmt-1::GFP + unc-119(+)]. Superficially wild-type. GFP expression in Pharynx and body wall muscles. Reference: Schmeisser K, et al. Nat Chem Biol. 2013 Sep 29. doi: 10.1038/nchembio.1352.
This laboratory hasn't submitted any alleles to the CGC.