Strain Information

Name MIR250   View On Wormbase
Species C. elegans
Genotypehif-1(ia4) V; risIs33.
DescriptionrisIs33 [K03A1.5p::3xFLAG::SV40-NLS::dCas9::SV40-NLS::VP64::HA + unc-119(+)]. risIs33 transgene stably expresses a 171 kDa dCas9::VP64 fusion protein suitable for for CRISPR activation (CRISPRa) in C. elegans, as described in Fischer F, et al. J Biol Chem. 2022 May 27;102085. doi: 10.1016/j.jbc.2022.102085. PMID: 35636511. Derived by crossing parental strains MIR249 and ZG31.
MutagenNo mutagen
Made byFabian Fischer
Laboratory MIR
Reference Fischer F, Benner C, Goyala A, Vitiello D, Grigolon G, Wu JY, Zarse K, Collin YE, Ristow M. Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation. J Biol Chem, doi: 10.1016/j.jbc.2022.102085 (2022) PMID: 35636511 DOI: 10.1016/j.jbc.2022.102085
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