Strain Information
| Name | LP515 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | cpIs89 I; cpIs85 II; egl-20(cp221[egl-20::mNG::3xFlag]) IV. |
| Description | cpIs89 [wrt-2p::2x mTurquoise2::PH::tbb-2 3'UTR loxN] I. cpIs85 [egl-20p::2x mKate2::PH::3xHA::tbb-2 3'UTR loxN] II. mNeonGreen::3xFlag tag inserted at the C-terminus of the endogenous egl-20 locus. 2x mTurquoise2::PH membrane marker expressed in seam cells, Q neuroblasts, and many hypodermal cells. Expression of 2x mKate2::PH membrane marker driven by egl-20 upstream intergenic sequence. cpIs89 is a single copy transgene inserted at Chr I:2851088 near ttTi4348 using Cas9-triggered homologous recombination. cpIs85 is a single copy transgene inserted at Chr II:8420157-8420243 near ttTi5605 using Cas9-triggered homologous recombination. Reference: Gibney TV & Pani AM. Development. 2025 Aug 21:dev.204802. doi: 10.1242/dev.204802. PMID: 40838367. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Ariel Pani |
| Laboratory | LP |
| Reference | Pani AM, Goldstein B. Direct visualization of a native Wnt in vivo reveals that a long-range Wnt gradient forms by extracellular dispersal. Elife. 2018 Aug 15;7:e38325. doi: 10.7554/eLife.38325. PMID: 30106379 |
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